Abstract
The somatic stem cells in the auditory organs can be utilized for the treatment of sensory neuronal hearing loss. The aim of this study was to isolate and culture of adult stem cells from guinea pig spiral ganglion in order to investigate the presence of soamtic stem cells in auditory organs of adult mammals. Spiral ganglions were obtained from the cochlea of guinea pigs, which weigh about 300 g. The tissues were dissociated and cultured in a medium added with epidermal growth factor and fibroblast growth factor. Then they were labeled with many markers for the stem cells and their differentiated cells, and identified under immunofluorecence microscope to find out the presence of somatic stem cells. The cultured cells can differentiate into neuronal cells positive for β III tubulin and glial cells positive for S-100. They were confirmed to be differentiated cells from neurosphere as the positive results for known stem cell marker such as bromodeoxyuridine(BrdU) and nestin. It was confirmed that the neural stem cells were existed in the spiral ganglion of guinea pig, which have the capability of self renewal and differentiation into specific neural cells. This fact presents a possibility of stem cell treatment for the patients of hearing impairment.
| Original language | English |
|---|---|
| Pages (from-to) | 938-941 |
| Number of pages | 4 |
| Journal | Tissue Engineering and Regenerative Medicine |
| Volume | 6 |
| Issue number | 4-11 |
| State | Published - Jun 2009 |
Keywords
- Neural
- Spiral ganglion
- Stem cell