Knockdown of 14-3-3ζ enhances radiosensitivity and radio-induced apoptosis in CD133 liver cancer stem cells

Young Ki Lee; Wonhee Hur; Sung Won Lee; Sung Woo Hong; Sung Woo Kim; Jung Eun Choi; Seung Kew Yoon

doi:10.1038/emm.2013.151

Knockdown of 14-3-3ζ enhances radiosensitivity and radio-induced apoptosis in CD133 liver cancer stem cells

Young Ki Lee
, Wonhee Hur
, Sung Won Lee
, Sung Woo Hong
, Sung Woo Kim
, Jung Eun Choi
, Seung Kew Yoon

The Catholic University of Korea

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

14-3-3ζ is related to many cancer survival cellular processes. In a previous study, we showed that silencing 14-3-3ζ decreases the resistance of hepatocellular carcinoma (HCC) to chemotherapy. In this study, we investigated whether silencing 14-3-3ζ affects the radioresistance of cancer stem-like cells (CSCs) in HCC. Knockdown of 14-3-3ζ decreased cell viability and the number of spheres by reducing radioresistance in CSCs after γ-irradiation (IR). Furthermore, the levels of pro-apoptotic proteins were upregulated in CSCs via silencing 14-3-3ζ after IR. These results suggest that 14-3-3ζ knockdown enhances radio-induced apoptosis by reducing radioresistance in liver CSCs. Experimental & Molecular Medicine (2014) 46, e77; doi:10.1038/emm.2013.151; published online 21 February 2014.

Original language	English
Article number	e77
Journal	Experimental and Molecular Medicine
Volume	46
Issue number	2
DOIs	https://doi.org/10.1038/emm.2013.151
State	Published - Feb 2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

14-3-3ζ
Apoptosis
Cancer stem-like cell
Hepatocellular carcinoma
Radioresistance

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10.1038/emm.2013.151

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title = "Knockdown of 14-3-3ζ enhances radiosensitivity and radio-induced apoptosis in CD133 liver cancer stem cells",

abstract = "14-3-3ζ is related to many cancer survival cellular processes. In a previous study, we showed that silencing 14-3-3ζ decreases the resistance of hepatocellular carcinoma (HCC) to chemotherapy. In this study, we investigated whether silencing 14-3-3ζ affects the radioresistance of cancer stem-like cells (CSCs) in HCC. Knockdown of 14-3-3ζ decreased cell viability and the number of spheres by reducing radioresistance in CSCs after γ-irradiation (IR). Furthermore, the levels of pro-apoptotic proteins were upregulated in CSCs via silencing 14-3-3ζ after IR. These results suggest that 14-3-3ζ knockdown enhances radio-induced apoptosis by reducing radioresistance in liver CSCs. Experimental \& Molecular Medicine (2014) 46, e77; doi:10.1038/emm.2013.151; published online 21 February 2014.",

keywords = "14-3-3ζ, Apoptosis, Cancer stem-like cell, Hepatocellular carcinoma, Radioresistance",

author = "Lee, \{Young Ki\} and Wonhee Hur and Lee, \{Sung Won\} and Hong, \{Sung Woo\} and Kim, \{Sung Woo\} and Choi, \{Jung Eun\} and Yoon, \{Seung Kew\}",

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doi = "10.1038/emm.2013.151",

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volume = "46",

journal = "Experimental and Molecular Medicine",

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AU - Lee, Young Ki

AU - Hur, Wonhee

AU - Lee, Sung Won

AU - Hong, Sung Woo

AU - Kim, Sung Woo

AU - Choi, Jung Eun

AU - Yoon, Seung Kew

PY - 2014/2

Y1 - 2014/2

N2 - 14-3-3ζ is related to many cancer survival cellular processes. In a previous study, we showed that silencing 14-3-3ζ decreases the resistance of hepatocellular carcinoma (HCC) to chemotherapy. In this study, we investigated whether silencing 14-3-3ζ affects the radioresistance of cancer stem-like cells (CSCs) in HCC. Knockdown of 14-3-3ζ decreased cell viability and the number of spheres by reducing radioresistance in CSCs after γ-irradiation (IR). Furthermore, the levels of pro-apoptotic proteins were upregulated in CSCs via silencing 14-3-3ζ after IR. These results suggest that 14-3-3ζ knockdown enhances radio-induced apoptosis by reducing radioresistance in liver CSCs. Experimental & Molecular Medicine (2014) 46, e77; doi:10.1038/emm.2013.151; published online 21 February 2014.

AB - 14-3-3ζ is related to many cancer survival cellular processes. In a previous study, we showed that silencing 14-3-3ζ decreases the resistance of hepatocellular carcinoma (HCC) to chemotherapy. In this study, we investigated whether silencing 14-3-3ζ affects the radioresistance of cancer stem-like cells (CSCs) in HCC. Knockdown of 14-3-3ζ decreased cell viability and the number of spheres by reducing radioresistance in CSCs after γ-irradiation (IR). Furthermore, the levels of pro-apoptotic proteins were upregulated in CSCs via silencing 14-3-3ζ after IR. These results suggest that 14-3-3ζ knockdown enhances radio-induced apoptosis by reducing radioresistance in liver CSCs. Experimental & Molecular Medicine (2014) 46, e77; doi:10.1038/emm.2013.151; published online 21 February 2014.

KW - 14-3-3ζ

KW - Apoptosis

KW - Cancer stem-like cell

KW - Hepatocellular carcinoma

KW - Radioresistance

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U2 - 10.1038/emm.2013.151

DO - 10.1038/emm.2013.151

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ER -

Knockdown of 14-3-3ζ enhances radiosensitivity and radio-induced apoptosis in CD133 liver cancer stem cells

Abstract

UN SDGs

Keywords

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