Light microscopic evidence of in vivo differentiation from the transplanted inferior turbinate-derived stem cell into the rod photoreceptor in degenerating retina of the mouse

Yong Soo Park; Yeonji Kim; Sung Won Kim; In Beom Kim

doi:10.1186/s42649-020-00031-w

Light microscopic evidence of in vivo differentiation from the transplanted inferior turbinate-derived stem cell into the rod photoreceptor in degenerating retina of the mouse

Yong Soo Park, Yeonji Kim, Sung Won Kim, In Beom Kim

Research output: Contribution to journal › Article › peer-review

Abstract

The human turbinate-derived mesenchymal stem cells (hTMSCs), which were DiI-labeled and transplanted into the subretinal space in degenerating mouse retina, were observed in retinal vertical sections processed for rhodopsin (a marker for rod photoreceptor) by confocal microscope with differential interference contrast (DIC) filters. The images clearly demonstrated that DiI-labeled hTMSCs have rhodopsin-immunoreactive appendages, indicating differentiation of transplanted hTMSC into rod photoreceptor. Conclusively, the finding suggests therapeutic potential of hTMSCs in retinal degeneration.

Original language	English
Article number	11
Journal	Applied Microscopy
Volume	50
Issue number	1
DOIs	https://doi.org/10.1186/s42649-020-00031-w
State	Published - Dec 2020

Bibliographical note

Funding Information:
This work was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI15C3076) and a grant of the Dios Pharma R&D 2019 Project.

Publisher Copyright:
© 2020, The Author(s).

Keywords

Confocal microscope
Differential interference contrast
Human turbinate-derived mesenchymal stem cell
Retinal degeneration
Rod photoreceptor

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10.1186/s42649-020-00031-w

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title = "Light microscopic evidence of in vivo differentiation from the transplanted inferior turbinate-derived stem cell into the rod photoreceptor in degenerating retina of the mouse",

abstract = "The human turbinate-derived mesenchymal stem cells (hTMSCs), which were DiI-labeled and transplanted into the subretinal space in degenerating mouse retina, were observed in retinal vertical sections processed for rhodopsin (a marker for rod photoreceptor) by confocal microscope with differential interference contrast (DIC) filters. The images clearly demonstrated that DiI-labeled hTMSCs have rhodopsin-immunoreactive appendages, indicating differentiation of transplanted hTMSC into rod photoreceptor. Conclusively, the finding suggests therapeutic potential of hTMSCs in retinal degeneration.",

keywords = "Confocal microscope, Differential interference contrast, Human turbinate-derived mesenchymal stem cell, Retinal degeneration, Rod photoreceptor",

author = "Park, {Yong Soo} and Yeonji Kim and Kim, {Sung Won} and Kim, {In Beom}",

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AU - Kim, In Beom

N1 - Funding Information: This work was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI15C3076) and a grant of the Dios Pharma R&D 2019 Project. Publisher Copyright: © 2020, The Author(s).

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AB - The human turbinate-derived mesenchymal stem cells (hTMSCs), which were DiI-labeled and transplanted into the subretinal space in degenerating mouse retina, were observed in retinal vertical sections processed for rhodopsin (a marker for rod photoreceptor) by confocal microscope with differential interference contrast (DIC) filters. The images clearly demonstrated that DiI-labeled hTMSCs have rhodopsin-immunoreactive appendages, indicating differentiation of transplanted hTMSC into rod photoreceptor. Conclusively, the finding suggests therapeutic potential of hTMSCs in retinal degeneration.

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Light microscopic evidence of in vivo differentiation from the transplanted inferior turbinate-derived stem cell into the rod photoreceptor in degenerating retina of the mouse

Abstract

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Keywords

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