Liquid chromatography-mass spectrometric analysis of compound K, a ginseng saponin metabolite, in rat plasma

Hye Young Ji; Hye Won Lee; Hui Hyun Kim; Hae Kyoung Kim; Yoon Chul Kim; Dong Hwan Sohn; Jaec Baek Kim; Hye Suk Lee

doi:10.1081/AL-120035900

Liquid chromatography-mass spectrometric analysis of compound K, a ginseng saponin metabolite, in rat plasma

Hye Young Ji, Hye Won Lee, Hui Hyun Kim, Hae Kyoung Kim, Yoon Chul Kim, Dong Hwan Sohn, Jaec Baek Kim, Hye Suk Lee

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A rapid, sensitive, and selective liquid chromatography-mass spectrometric (LC/MS) method for the determination of compound K, a ginseng saponin metabolite, in rat plasma was developed. Compound K and internal standard, lithospermic acid B, dimethylester were extracted from rat plasma by liquid-liquid extraction and analyzed on Atlantis column with the mobile phase of acetonitrile-ammonium formate (10 mM, pH 3.0) (75: 25, v/v). The analytes were detected using an electrospray negative ionization mass spectrometry in the selected ion monitoring (SIR) mode. The standard curve was linear (r ² = 0.998) over the concentration range of 2.0-500 ng/mL. The relative standard deviation (RSD) of intra- and inter-assay ranged from 1.2-3. 0% to 1.3-2.9%, respectively. The recoveries of compound K ranged from 97.7% to 98.9%, with that of lithospermic acid B, dimethylester (internal standard) being 96.6% + 7.4%. The lower limit of quantification for compound K was 2.0 ng/mL using 100 μL of plasma sample. This method was successfully applied to the pharmacokinetic study of compound K in rats.

Original language	English
Pages (from-to)	1307-1318
Number of pages	12
Journal	Analytical Letters
Volume	37
Issue number	7
DOIs	https://doi.org/10.1081/AL-120035900
State	Published - 2004

Bibliographical note

Funding Information:
This research was supported by a grant (PF 0320401-00) from Plant Diversity Research Center of 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korea government and Spela Ltd.

Keywords

Compound K
Ginseng saponin metabolite
LC/MS
Plasma

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10.1081/AL-120035900

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title = "Liquid chromatography-mass spectrometric analysis of compound K, a ginseng saponin metabolite, in rat plasma",

abstract = "A rapid, sensitive, and selective liquid chromatography-mass spectrometric (LC/MS) method for the determination of compound K, a ginseng saponin metabolite, in rat plasma was developed. Compound K and internal standard, lithospermic acid B, dimethylester were extracted from rat plasma by liquid-liquid extraction and analyzed on Atlantis column with the mobile phase of acetonitrile-ammonium formate (10 mM, pH 3.0) (75: 25, v/v). The analytes were detected using an electrospray negative ionization mass spectrometry in the selected ion monitoring (SIR) mode. The standard curve was linear (r 2 = 0.998) over the concentration range of 2.0-500 ng/mL. The relative standard deviation (RSD) of intra- and inter-assay ranged from 1.2-3. 0% to 1.3-2.9%, respectively. The recoveries of compound K ranged from 97.7% to 98.9%, with that of lithospermic acid B, dimethylester (internal standard) being 96.6% + 7.4%. The lower limit of quantification for compound K was 2.0 ng/mL using 100 μL of plasma sample. This method was successfully applied to the pharmacokinetic study of compound K in rats.",

keywords = "Compound K, Ginseng saponin metabolite, LC/MS, Plasma",

author = "Ji, {Hye Young} and Lee, {Hye Won} and Kim, {Hui Hyun} and Kim, {Hae Kyoung} and Kim, {Yoon Chul} and Sohn, {Dong Hwan} and Kim, {Jaec Baek} and Lee, {Hye Suk}",

note = "Funding Information: This research was supported by a grant (PF 0320401-00) from Plant Diversity Research Center of 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korea government and Spela Ltd.",

year = "2004",

doi = "10.1081/AL-120035900",

language = "English",

volume = "37",

pages = "1307--1318",

journal = "Analytical Letters",

issn = "0003-2719",

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T1 - Liquid chromatography-mass spectrometric analysis of compound K, a ginseng saponin metabolite, in rat plasma

AU - Ji, Hye Young

AU - Lee, Hye Won

AU - Kim, Hui Hyun

AU - Kim, Hae Kyoung

AU - Kim, Yoon Chul

AU - Sohn, Dong Hwan

AU - Kim, Jaec Baek

AU - Lee, Hye Suk

N1 - Funding Information: This research was supported by a grant (PF 0320401-00) from Plant Diversity Research Center of 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korea government and Spela Ltd.

PY - 2004

Y1 - 2004

N2 - A rapid, sensitive, and selective liquid chromatography-mass spectrometric (LC/MS) method for the determination of compound K, a ginseng saponin metabolite, in rat plasma was developed. Compound K and internal standard, lithospermic acid B, dimethylester were extracted from rat plasma by liquid-liquid extraction and analyzed on Atlantis column with the mobile phase of acetonitrile-ammonium formate (10 mM, pH 3.0) (75: 25, v/v). The analytes were detected using an electrospray negative ionization mass spectrometry in the selected ion monitoring (SIR) mode. The standard curve was linear (r 2 = 0.998) over the concentration range of 2.0-500 ng/mL. The relative standard deviation (RSD) of intra- and inter-assay ranged from 1.2-3. 0% to 1.3-2.9%, respectively. The recoveries of compound K ranged from 97.7% to 98.9%, with that of lithospermic acid B, dimethylester (internal standard) being 96.6% + 7.4%. The lower limit of quantification for compound K was 2.0 ng/mL using 100 μL of plasma sample. This method was successfully applied to the pharmacokinetic study of compound K in rats.

AB - A rapid, sensitive, and selective liquid chromatography-mass spectrometric (LC/MS) method for the determination of compound K, a ginseng saponin metabolite, in rat plasma was developed. Compound K and internal standard, lithospermic acid B, dimethylester were extracted from rat plasma by liquid-liquid extraction and analyzed on Atlantis column with the mobile phase of acetonitrile-ammonium formate (10 mM, pH 3.0) (75: 25, v/v). The analytes were detected using an electrospray negative ionization mass spectrometry in the selected ion monitoring (SIR) mode. The standard curve was linear (r 2 = 0.998) over the concentration range of 2.0-500 ng/mL. The relative standard deviation (RSD) of intra- and inter-assay ranged from 1.2-3. 0% to 1.3-2.9%, respectively. The recoveries of compound K ranged from 97.7% to 98.9%, with that of lithospermic acid B, dimethylester (internal standard) being 96.6% + 7.4%. The lower limit of quantification for compound K was 2.0 ng/mL using 100 μL of plasma sample. This method was successfully applied to the pharmacokinetic study of compound K in rats.

KW - Compound K

KW - Ginseng saponin metabolite

KW - LC/MS

KW - Plasma

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U2 - 10.1081/AL-120035900

DO - 10.1081/AL-120035900

M3 - Article

AN - SCOPUS:2942594000

SN - 0003-2719

VL - 37

SP - 1307

EP - 1318

JO - Analytical Letters

JF - Analytical Letters

IS - 7

ER -

Liquid chromatography-mass spectrometric analysis of compound K, a ginseng saponin metabolite, in rat plasma

Abstract

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Keywords

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