Magnetic resonance tracking of multifunctional nanoparticle-labeled mouse mesenchymal stem cells in a mouse model of myocardial infarction

Kyuhong Lee; Cheongsoo Park; Hye Young Moon; Eunjung Ahn; Hyo Eun Park; Sang Hyun Ihm; Ki Bae Seung; Tae Jong Yoon; Kiyuk Chang; Chulhyun Lee; Chaejoon Cheong; Kwan Soo Hong

doi:10.1016/j.cap.2008.08.009

Magnetic resonance tracking of multifunctional nanoparticle-labeled mouse mesenchymal stem cells in a mouse model of myocardial infarction

Kyuhong Lee
, Cheongsoo Park
, Hye Young Moon
, Eunjung Ahn
, Hyo Eun Park
, Sang Hyun Ihm
, Ki Bae Seung
, Tae Jong Yoon
, Kiyuk Chang
, Chulhyun Lee
, Chaejoon Cheong
, Kwan Soo Hong

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Magnetic ferrite nanoparticles (MNPs) are nano-sized magnets that distort the local magnetic field and alter the T₂ and T₂^* of the water protons in the surrounding water molecules. This property renders MNPs a favorable negative MR contrast agent for biomedical applications. Silica coating and polyethylene glycolation confers additional stability and biocompatibility to the MNPs. Organic dyes incorporated into the silica shell enable the detection of MNP fluorescence by confocal laser scanning microscopy (CLSM). Mouse mesenchymal stem cells (mMSCs) in adult bone marrow can differentiate into cardiomyocytes. The mMSCs recruited in the region of myocardial infarction can express muscle-specific markers and increase the regional wall motion. Using MRI, we tracked the fate of MNP-labeled mMSCs injected into the infarcted myocardium. The mMSCs were effectively labeled with polyethylene-glycolated MNPs, as confirmed by CLSM. The MNP-labeled mMSCs were injected around and into the infarcted myocardium of C57/BL6 mice. Using MR, we successfully tracked the MNP-labeled mMSCs in the hearts of mice at 7 d after inducing myocardial infarction. The localization of MNPs in the myocardium was validated by the in vivo and ex vivo MR images.

Original language	English
Pages (from-to)	S12-S14
Journal	Current Applied Physics
Volume	9
Issue number	1 SUPPL.
DOIs	https://doi.org/10.1016/j.cap.2008.08.009
State	Published - Jan 2009

Bibliographical note

Funding Information:
We wish to acknowledge the financial supports provided by the KBSI Grant (T27041) to KSH and by the Catholic Institute of Cell Therapy Basic Science Programs Foundation established in 2006.

Keywords

MRI
Myocardial infarction
Nanoparticles
Stem cell

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10.1016/j.cap.2008.08.009

Cite this

Lee, K., Park, C., Moon, H. Y., Ahn, E., Park, H. E., Ihm, S. H., Seung, K. B., Yoon, T. J., Chang, K., Lee, C., Cheong, C., & Hong, K. S. (2009). Magnetic resonance tracking of multifunctional nanoparticle-labeled mouse mesenchymal stem cells in a mouse model of myocardial infarction. Current Applied Physics, 9(1 SUPPL.), S12-S14. https://doi.org/10.1016/j.cap.2008.08.009

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title = "Magnetic resonance tracking of multifunctional nanoparticle-labeled mouse mesenchymal stem cells in a mouse model of myocardial infarction",

abstract = "Magnetic ferrite nanoparticles (MNPs) are nano-sized magnets that distort the local magnetic field and alter the T2 and T2* of the water protons in the surrounding water molecules. This property renders MNPs a favorable negative MR contrast agent for biomedical applications. Silica coating and polyethylene glycolation confers additional stability and biocompatibility to the MNPs. Organic dyes incorporated into the silica shell enable the detection of MNP fluorescence by confocal laser scanning microscopy (CLSM). Mouse mesenchymal stem cells (mMSCs) in adult bone marrow can differentiate into cardiomyocytes. The mMSCs recruited in the region of myocardial infarction can express muscle-specific markers and increase the regional wall motion. Using MRI, we tracked the fate of MNP-labeled mMSCs injected into the infarcted myocardium. The mMSCs were effectively labeled with polyethylene-glycolated MNPs, as confirmed by CLSM. The MNP-labeled mMSCs were injected around and into the infarcted myocardium of C57/BL6 mice. Using MR, we successfully tracked the MNP-labeled mMSCs in the hearts of mice at 7 d after inducing myocardial infarction. The localization of MNPs in the myocardium was validated by the in vivo and ex vivo MR images.",

keywords = "MRI, Myocardial infarction, Nanoparticles, Stem cell",

author = "Kyuhong Lee and Cheongsoo Park and Moon, \{Hye Young\} and Eunjung Ahn and Park, \{Hyo Eun\} and Ihm, \{Sang Hyun\} and Seung, \{Ki Bae\} and Yoon, \{Tae Jong\} and Kiyuk Chang and Chulhyun Lee and Chaejoon Cheong and Hong, \{Kwan Soo\}",

note = "Funding Information: We wish to acknowledge the financial supports provided by the KBSI Grant (T27041) to KSH and by the Catholic Institute of Cell Therapy Basic Science Programs Foundation established in 2006.",

year = "2009",

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doi = "10.1016/j.cap.2008.08.009",

language = "English",

volume = "9",

pages = "S12--S14",

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AU - Lee, Kyuhong

AU - Park, Cheongsoo

AU - Moon, Hye Young

AU - Ahn, Eunjung

AU - Park, Hyo Eun

AU - Ihm, Sang Hyun

AU - Seung, Ki Bae

AU - Yoon, Tae Jong

AU - Chang, Kiyuk

AU - Lee, Chulhyun

AU - Cheong, Chaejoon

AU - Hong, Kwan Soo

N1 - Funding Information: We wish to acknowledge the financial supports provided by the KBSI Grant (T27041) to KSH and by the Catholic Institute of Cell Therapy Basic Science Programs Foundation established in 2006.

PY - 2009/1

Y1 - 2009/1

N2 - Magnetic ferrite nanoparticles (MNPs) are nano-sized magnets that distort the local magnetic field and alter the T2 and T2* of the water protons in the surrounding water molecules. This property renders MNPs a favorable negative MR contrast agent for biomedical applications. Silica coating and polyethylene glycolation confers additional stability and biocompatibility to the MNPs. Organic dyes incorporated into the silica shell enable the detection of MNP fluorescence by confocal laser scanning microscopy (CLSM). Mouse mesenchymal stem cells (mMSCs) in adult bone marrow can differentiate into cardiomyocytes. The mMSCs recruited in the region of myocardial infarction can express muscle-specific markers and increase the regional wall motion. Using MRI, we tracked the fate of MNP-labeled mMSCs injected into the infarcted myocardium. The mMSCs were effectively labeled with polyethylene-glycolated MNPs, as confirmed by CLSM. The MNP-labeled mMSCs were injected around and into the infarcted myocardium of C57/BL6 mice. Using MR, we successfully tracked the MNP-labeled mMSCs in the hearts of mice at 7 d after inducing myocardial infarction. The localization of MNPs in the myocardium was validated by the in vivo and ex vivo MR images.

AB - Magnetic ferrite nanoparticles (MNPs) are nano-sized magnets that distort the local magnetic field and alter the T2 and T2* of the water protons in the surrounding water molecules. This property renders MNPs a favorable negative MR contrast agent for biomedical applications. Silica coating and polyethylene glycolation confers additional stability and biocompatibility to the MNPs. Organic dyes incorporated into the silica shell enable the detection of MNP fluorescence by confocal laser scanning microscopy (CLSM). Mouse mesenchymal stem cells (mMSCs) in adult bone marrow can differentiate into cardiomyocytes. The mMSCs recruited in the region of myocardial infarction can express muscle-specific markers and increase the regional wall motion. Using MRI, we tracked the fate of MNP-labeled mMSCs injected into the infarcted myocardium. The mMSCs were effectively labeled with polyethylene-glycolated MNPs, as confirmed by CLSM. The MNP-labeled mMSCs were injected around and into the infarcted myocardium of C57/BL6 mice. Using MR, we successfully tracked the MNP-labeled mMSCs in the hearts of mice at 7 d after inducing myocardial infarction. The localization of MNPs in the myocardium was validated by the in vivo and ex vivo MR images.

KW - MRI

KW - Myocardial infarction

KW - Nanoparticles

KW - Stem cell

UR - https://www.scopus.com/pages/publications/55649111331

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DO - 10.1016/j.cap.2008.08.009

M3 - Article

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SN - 1567-1739

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SP - S12-S14

JO - Current Applied Physics

JF - Current Applied Physics

IS - 1 SUPPL.

ER -

Magnetic resonance tracking of multifunctional nanoparticle-labeled mouse mesenchymal stem cells in a mouse model of myocardial infarction

Abstract

Bibliographical note

Keywords

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