Abstract
Magnolin, a bioactive lignan separated from Flos Magnoliae, possesses anti-inflammatory, anticancer, antioxidant, and vasodilator effects. This study compared the metabolic profiles of magnolin in human and rat hepatocytes using liquid chromatography-high resolution mass spectrometry. The hepatic extraction ratio of magnolin in human and rat hepatocytes were 0.27 and 0.14, respectively, suggesting that it undergoes a low-to-moderate degree of hepatic metabolism. During metabolism in human hepatocytes, magnolin generated 8 phase 1 metabolites, including O-desmethylmagnolin (M1-M4), hydroxymagnolin (M5), di-O-desmethylmagnolin (M6 and M7), and hydroxy-O-desmethylmagnolin (M8), and 11 phase 2 metabolites, including glucuronides of M1-M4 (M9-M12) and M7 (M15), sulfates of M1, M2, M6, and M7 (M13, M14, and M16-M18), and M6 disulfate (M19). However, 6 phase 1 metabolites (M1-M5 and M8) and 6 phase 2 metabolites (M9-M14) were formed after incubation of magnolin with rat hepatocytes. These findings help to predict the pharmacokinetics and metabolism of magnolin in humans.
| Original language | English |
|---|---|
| Pages (from-to) | 202-210 |
| Number of pages | 9 |
| Journal | Mass Spectrometry Letters |
| Volume | 15 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 2024 |
Bibliographical note
Publisher Copyright:© Korean Society for Mass Spectrometry.
Keywords
- LC-HRMS
- hepatocytes
- in vitro metabolism
- magnolin
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