Monitoring the antiviral effect of alpha interferon on individual cells

Saeng Kim Chon; Ha Jung Jong; Takaji Wakita; Kew Yoon Seung; Key Jang Sung

doi:10.1128/JVI.02824-06

Monitoring the antiviral effect of alpha interferon on individual cells

Saeng Kim Chon
, Ha Jung Jong
, Takaji Wakita
, Kew Yoon Seung
, Key Jang Sung

Division of Gastroenterology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

An infectious hepatitis C virus (HCV) cDNA clone (JFH1) was generated recently. However, quantitative analysis of HCV infection and observation of infected cells have proved to be difficult because the yield of HCV in cell cultures is fairly low. We generated infectious HCV clones containing the convenient reporters green fluorescent protein (GFP) and Renilla luciferase in the NS5a-coding sequence. The new viruses responded to antiviral agents in a dose-dependent manner. Responses of individual cells containing HCV to alpha interferon (IFN-α) were monitored using GFP-tagged HCV and time-lapse confocal microscopy. Marked variations in the response to IFN-α were observed among HCV-containing cells.

Original language	English
Pages (from-to)	8814-8820
Number of pages	7
Journal	Journal of Virology
Volume	81
Issue number	16
DOIs	https://doi.org/10.1128/JVI.02824-06
State	Published - Aug 2007

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10.1128/JVI.02824-06

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title = "Monitoring the antiviral effect of alpha interferon on individual cells",

abstract = "An infectious hepatitis C virus (HCV) cDNA clone (JFH1) was generated recently. However, quantitative analysis of HCV infection and observation of infected cells have proved to be difficult because the yield of HCV in cell cultures is fairly low. We generated infectious HCV clones containing the convenient reporters green fluorescent protein (GFP) and Renilla luciferase in the NS5a-coding sequence. The new viruses responded to antiviral agents in a dose-dependent manner. Responses of individual cells containing HCV to alpha interferon (IFN-α) were monitored using GFP-tagged HCV and time-lapse confocal microscopy. Marked variations in the response to IFN-α were observed among HCV-containing cells.",

author = "Chon, \{Saeng Kim\} and Jong, \{Ha Jung\} and Takaji Wakita and Seung, \{Kew Yoon\} and Sung, \{Key Jang\}",

year = "2007",

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language = "English",

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T1 - Monitoring the antiviral effect of alpha interferon on individual cells

AU - Chon, Saeng Kim

AU - Jong, Ha Jung

AU - Wakita, Takaji

AU - Seung, Kew Yoon

AU - Sung, Key Jang

PY - 2007/8

Y1 - 2007/8

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AB - An infectious hepatitis C virus (HCV) cDNA clone (JFH1) was generated recently. However, quantitative analysis of HCV infection and observation of infected cells have proved to be difficult because the yield of HCV in cell cultures is fairly low. We generated infectious HCV clones containing the convenient reporters green fluorescent protein (GFP) and Renilla luciferase in the NS5a-coding sequence. The new viruses responded to antiviral agents in a dose-dependent manner. Responses of individual cells containing HCV to alpha interferon (IFN-α) were monitored using GFP-tagged HCV and time-lapse confocal microscopy. Marked variations in the response to IFN-α were observed among HCV-containing cells.

UR - https://www.scopus.com/pages/publications/34547788145

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DO - 10.1128/JVI.02824-06

M3 - Article

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AN - SCOPUS:34547788145

SN - 0022-538X

VL - 81

SP - 8814

EP - 8820

JO - Journal of Virology

JF - Journal of Virology

IS - 16

ER -

Monitoring the antiviral effect of alpha interferon on individual cells

Abstract

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