Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro

Abu Saleh Md Moin; Hideki Yamaguchi; Marie Rhee; Ji Wom Kim; Koji Toshinai; T. M.Zaved Waise; Farhana Naznin; Takashi Matsuo; Kazuki Sasaki; Naoto Minamino; Kun Ho Yoon; Masamitsu Nakazato

doi:10.1016/j.bbrc.2012.10.073

Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro

Abu Saleh Md Moin, Hideki Yamaguchi, Marie Rhee, Ji Wom Kim, Koji Toshinai, T. M.Zaved Waise, Farhana Naznin, Takashi Matsuo, Kazuki Sasaki, Naoto Minamino, Kun Ho Yoon, Masamitsu Nakazato

Division of Endocrinology & Metabolism

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Neuroendocrine regulatory peptide (NERP)-2, recently identified as a bioactive peptide involved in vasopressin secretion and feeding regulation in the central nervous system, is abundantly expressed in endocrine cells in peripheral tissues. To explore the physiological roles of NERP-2 in the pancreas, we examined its effects on insulin secretion. NERP-2 increased glucose-stimulated insulin secretion (GSIS) in a dose-dependent manner, with a lowest effective dose of 10^-7M, from the pancreatic β-cell line MIN6 and isolated mouse pancreatic islets. NERP-2 did not affect insulin secretion under the low-glucose conditions. Neither NERP-1 nor NERP-2-Gly (nonamidated NERP-2) stimulated insulin secretion. NERP-2 significantly augmented GSIS after intravenous administration to anesthetized rats or intraperitoneal injection to conscious mice. We detected NERP-2 in pancreatic islets, where it co-localized extensively with insulin. Calcium-imaging analysis demonstrated that NERP-2 increased the calcium influx in MIN6 cells. These findings reveal that NERP-2 regulates GSIS by elevating intracellular calcium concentrations.

Original language	English
Pages (from-to)	512-517
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	428
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2012.10.073
State	Published - 30 Nov 2012

Bibliographical note

Funding Information:
We thank R. Matsuura for expert technical assistance. This work was supported in part by JSPS KAKENHI (No. 23591358 ) to H.Y.; and MEXT KAKENHI (No. 22126009 ), JSPS KAKENHI (No. 22390190 ), grants from Takeda Science Foundation , and Strategic International Research Cooperative Program, Japan Science and Technology Agency (JST) to M.N.

Keywords

Ca
DAB
GSIS
Insulin secretion
NERP
NERP-2
VGF

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Moin, A. S. M., Yamaguchi, H., Rhee, M., Kim, J. W., Toshinai, K., Waise, T. M. Z., Naznin, F., Matsuo, T., Sasaki, K., Minamino, N., Yoon, K. H., & Nakazato, M. (2012). Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro. Biochemical and Biophysical Research Communications, 428(4), 512-517. https://doi.org/10.1016/j.bbrc.2012.10.073

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title = "Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro",

abstract = "Neuroendocrine regulatory peptide (NERP)-2, recently identified as a bioactive peptide involved in vasopressin secretion and feeding regulation in the central nervous system, is abundantly expressed in endocrine cells in peripheral tissues. To explore the physiological roles of NERP-2 in the pancreas, we examined its effects on insulin secretion. NERP-2 increased glucose-stimulated insulin secretion (GSIS) in a dose-dependent manner, with a lowest effective dose of 10-7M, from the pancreatic β-cell line MIN6 and isolated mouse pancreatic islets. NERP-2 did not affect insulin secretion under the low-glucose conditions. Neither NERP-1 nor NERP-2-Gly (nonamidated NERP-2) stimulated insulin secretion. NERP-2 significantly augmented GSIS after intravenous administration to anesthetized rats or intraperitoneal injection to conscious mice. We detected NERP-2 in pancreatic islets, where it co-localized extensively with insulin. Calcium-imaging analysis demonstrated that NERP-2 increased the calcium influx in MIN6 cells. These findings reveal that NERP-2 regulates GSIS by elevating intracellular calcium concentrations.",

keywords = "Ca, DAB, GSIS, Insulin secretion, NERP, NERP-2, VGF",

author = "Moin, {Abu Saleh Md} and Hideki Yamaguchi and Marie Rhee and Kim, {Ji Wom} and Koji Toshinai and Waise, {T. M.Zaved} and Farhana Naznin and Takashi Matsuo and Kazuki Sasaki and Naoto Minamino and Yoon, {Kun Ho} and Masamitsu Nakazato",

note = "Funding Information: We thank R. Matsuura for expert technical assistance. This work was supported in part by JSPS KAKENHI (No. 23591358 ) to H.Y.; and MEXT KAKENHI (No. 22126009 ), JSPS KAKENHI (No. 22390190 ), grants from Takeda Science Foundation , and Strategic International Research Cooperative Program, Japan Science and Technology Agency (JST) to M.N.",

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doi = "10.1016/j.bbrc.2012.10.073",

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Moin, ASM, Yamaguchi, H, Rhee, M, Kim, JW, Toshinai, K, Waise, TMZ, Naznin, F, Matsuo, T, Sasaki, K, Minamino, N, Yoon, KH & Nakazato, M 2012, 'Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro', Biochemical and Biophysical Research Communications, vol. 428, no. 4, pp. 512-517. https://doi.org/10.1016/j.bbrc.2012.10.073

TY - JOUR

T1 - Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro

AU - Moin, Abu Saleh Md

AU - Yamaguchi, Hideki

AU - Rhee, Marie

AU - Kim, Ji Wom

AU - Toshinai, Koji

AU - Waise, T. M.Zaved

AU - Naznin, Farhana

AU - Matsuo, Takashi

AU - Sasaki, Kazuki

AU - Minamino, Naoto

AU - Yoon, Kun Ho

AU - Nakazato, Masamitsu

N1 - Funding Information: We thank R. Matsuura for expert technical assistance. This work was supported in part by JSPS KAKENHI (No. 23591358 ) to H.Y.; and MEXT KAKENHI (No. 22126009 ), JSPS KAKENHI (No. 22390190 ), grants from Takeda Science Foundation , and Strategic International Research Cooperative Program, Japan Science and Technology Agency (JST) to M.N.

PY - 2012/11/30

Y1 - 2012/11/30

N2 - Neuroendocrine regulatory peptide (NERP)-2, recently identified as a bioactive peptide involved in vasopressin secretion and feeding regulation in the central nervous system, is abundantly expressed in endocrine cells in peripheral tissues. To explore the physiological roles of NERP-2 in the pancreas, we examined its effects on insulin secretion. NERP-2 increased glucose-stimulated insulin secretion (GSIS) in a dose-dependent manner, with a lowest effective dose of 10-7M, from the pancreatic β-cell line MIN6 and isolated mouse pancreatic islets. NERP-2 did not affect insulin secretion under the low-glucose conditions. Neither NERP-1 nor NERP-2-Gly (nonamidated NERP-2) stimulated insulin secretion. NERP-2 significantly augmented GSIS after intravenous administration to anesthetized rats or intraperitoneal injection to conscious mice. We detected NERP-2 in pancreatic islets, where it co-localized extensively with insulin. Calcium-imaging analysis demonstrated that NERP-2 increased the calcium influx in MIN6 cells. These findings reveal that NERP-2 regulates GSIS by elevating intracellular calcium concentrations.

AB - Neuroendocrine regulatory peptide (NERP)-2, recently identified as a bioactive peptide involved in vasopressin secretion and feeding regulation in the central nervous system, is abundantly expressed in endocrine cells in peripheral tissues. To explore the physiological roles of NERP-2 in the pancreas, we examined its effects on insulin secretion. NERP-2 increased glucose-stimulated insulin secretion (GSIS) in a dose-dependent manner, with a lowest effective dose of 10-7M, from the pancreatic β-cell line MIN6 and isolated mouse pancreatic islets. NERP-2 did not affect insulin secretion under the low-glucose conditions. Neither NERP-1 nor NERP-2-Gly (nonamidated NERP-2) stimulated insulin secretion. NERP-2 significantly augmented GSIS after intravenous administration to anesthetized rats or intraperitoneal injection to conscious mice. We detected NERP-2 in pancreatic islets, where it co-localized extensively with insulin. Calcium-imaging analysis demonstrated that NERP-2 increased the calcium influx in MIN6 cells. These findings reveal that NERP-2 regulates GSIS by elevating intracellular calcium concentrations.

KW - Ca

KW - DAB

KW - GSIS

KW - Insulin secretion

KW - NERP

KW - NERP-2

KW - VGF

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U2 - 10.1016/j.bbrc.2012.10.073

DO - 10.1016/j.bbrc.2012.10.073

M3 - Article

C2 - 23111332

AN - SCOPUS:84870335269

SN - 0006-291X

VL - 428

SP - 512

EP - 517

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro

Abstract

Bibliographical note

Keywords

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