Novel genes and cellular pathways related to infection with adenovirus-36 as an obesity agent in human mesenchymal stem cells

H. N. Na; H. Kim; J. H. Nam

doi:10.1038/ijo.2011.89

Novel genes and cellular pathways related to infection with adenovirus-36 as an obesity agent in human mesenchymal stem cells

H. N. Na
, H. Kim
, J. H. Nam

Department of Medical and Biological Sciences

The Catholic University of Korea
Hanyang University

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Objective: The objective of this study was to investigate the molecular mechanisms underlying adenovirus-36 (Ad-36)-induced obesity by the identification of novel genes and cellular pathways. Design: Viral growth, intracellular lipid accumulation and gene expression profiles were determined in human mesenchymal stem cells (hMSCs) infected with Ad-36 or Ad-2. A microarray assay and gene set enrichment analysis (GSEA) were performed to assess alterations in global gene expression profiles. Results: Ad-36, but not Ad-2, induced lipid accumulation and upregulated adipogenesis-related genes. There was no difference in viral growth between Ad-36 infection and Ad-2 infection in hMSCs. GSEA revealed that Ad-36 infection was more frequently associated with activation of novel pathways, including the PPAR-gamma signaling pathway, and inflammation compared with Ad-2 infection, raising the possibility that these pathways may be key regulators of Ad-36-induced adipogenesis. Conclusion: This study may help foster a better understanding of the roles of several cellular factors in Ad-36-induced obesity.

Original language	English
Pages (from-to)	195-200
Number of pages	6
Journal	International Journal of Obesity
Volume	36
Issue number	2
DOIs	https://doi.org/10.1038/ijo.2011.89
State	Published - Feb 2012

Bibliographical note

Funding Information:
This study was supported by a grant from the GRRC of the Catholic University of Korea, the Biogreen21 Program (PJ007186) of Rural Development of Administration, MKE and KOTEF through the Human Resource Training Project for Strategic Technology, and Medical Research Center (2010-0029474) programs funded by the Ministry of Science and Technology, Republic of Korea. H-N Na was supported by Hi Seoul Science (Humanities) Fellowship funded by the Seoul Scholarship Foundation.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Ad-36
PPAR-gamma signaling
gene set enrichment analysis
human mesenchymal stem cell
inflammation

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10.1038/ijo.2011.89

Cite this

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title = "Novel genes and cellular pathways related to infection with adenovirus-36 as an obesity agent in human mesenchymal stem cells",

abstract = "Objective: The objective of this study was to investigate the molecular mechanisms underlying adenovirus-36 (Ad-36)-induced obesity by the identification of novel genes and cellular pathways. Design: Viral growth, intracellular lipid accumulation and gene expression profiles were determined in human mesenchymal stem cells (hMSCs) infected with Ad-36 or Ad-2. A microarray assay and gene set enrichment analysis (GSEA) were performed to assess alterations in global gene expression profiles. Results: Ad-36, but not Ad-2, induced lipid accumulation and upregulated adipogenesis-related genes. There was no difference in viral growth between Ad-36 infection and Ad-2 infection in hMSCs. GSEA revealed that Ad-36 infection was more frequently associated with activation of novel pathways, including the PPAR-gamma signaling pathway, and inflammation compared with Ad-2 infection, raising the possibility that these pathways may be key regulators of Ad-36-induced adipogenesis. Conclusion: This study may help foster a better understanding of the roles of several cellular factors in Ad-36-induced obesity.",

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author = "Na, \{H. N.\} and H. Kim and Nam, \{J. H.\}",

note = "Funding Information: This study was supported by a grant from the GRRC of the Catholic University of Korea, the Biogreen21 Program (PJ007186) of Rural Development of Administration, MKE and KOTEF through the Human Resource Training Project for Strategic Technology, and Medical Research Center (2010-0029474) programs funded by the Ministry of Science and Technology, Republic of Korea. H-N Na was supported by Hi Seoul Science (Humanities) Fellowship funded by the Seoul Scholarship Foundation.",

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doi = "10.1038/ijo.2011.89",

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AU - Kim, H.

AU - Nam, J. H.

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N2 - Objective: The objective of this study was to investigate the molecular mechanisms underlying adenovirus-36 (Ad-36)-induced obesity by the identification of novel genes and cellular pathways. Design: Viral growth, intracellular lipid accumulation and gene expression profiles were determined in human mesenchymal stem cells (hMSCs) infected with Ad-36 or Ad-2. A microarray assay and gene set enrichment analysis (GSEA) were performed to assess alterations in global gene expression profiles. Results: Ad-36, but not Ad-2, induced lipid accumulation and upregulated adipogenesis-related genes. There was no difference in viral growth between Ad-36 infection and Ad-2 infection in hMSCs. GSEA revealed that Ad-36 infection was more frequently associated with activation of novel pathways, including the PPAR-gamma signaling pathway, and inflammation compared with Ad-2 infection, raising the possibility that these pathways may be key regulators of Ad-36-induced adipogenesis. Conclusion: This study may help foster a better understanding of the roles of several cellular factors in Ad-36-induced obesity.

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Novel genes and cellular pathways related to infection with adenovirus-36 as an obesity agent in human mesenchymal stem cells

Abstract

Bibliographical note

UN SDGs

Keywords

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