Abstract
We investigated transesterification activity of methanol-stable lipase from Staphylococcus haemolyticus L62. Lipase L62 transesterified olive oil and menhaden oil using various alcohols including methanol, ethanol, 1-propanol, and 1-butanol. Based on the high stability of lipase L62 toward alcohols, high molar ratios (3:1 or 6:1) of alcohol to oil were employed in the reaction systems. Lipase L62 produced fatty acid esters efficiently by one-step transesterification. Reaction conditions for production of fatty acid methyl esters (FAMEs) and omega-3 fatty acid ethyl esters were further optimized. Lipase L62 produced those esters efficiently when 30–40 % water content was present in the reaction medium. The lipase L62 conversion yield was as high as 92 % for FAME and omega-3 ethyl ester production. These results indicate that lipase L62 is a suitable catalyst for producing an omega-3 ethyl ester concentrate in the pharmaceutical industry as well as for producing biodiesel in the bio-refinery industry.
| Original language | English |
|---|---|
| Pages (from-to) | 195-202 |
| Number of pages | 8 |
| Journal | Journal of the Korean Society for Applied Biological Chemistry |
| Volume | 58 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 2015 |
Bibliographical note
Publisher Copyright:© 2015, The Korean Society for Applied Biological Chemistry.
Keywords
- Biodiesel
- Lipase
- Omega-3 ethyl ester
- Staphylococcus haemolyticus L62
- Transesterification
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