Abstract
In this study, we evaluated the osteogenic potential and biomechnical properties of autologous cultured osteoblasts mixed with fibrin when transplanted to bone defects. Radial shaft defects greater than 15 mm were created in 14 New Zealand white rabbits. The defects were made in both radial bones of each rabbit. In these fourteen rabbits, the right-side defect was used as the experimental side and was filled with autologous cultured osteoblasts mixed with fibrin, while the left side defect was used as the control side and was filled only with fibrin. After eight weeks, both groups were compared regarding the area of callus formation and optical density of the callus as measured radiologically, the stiffness, and energy absorption measured biomechanically. There was no radiologic difference in the size of bone formation, however, the optical density of the newly formed bone of the experimental group was significantly higher than that of the control group(p<0.05). The stiffness on the experimental side was significantly higher than that on the control side(p<0.05). We hypothesize that autologous osteoblast cells mixed with fibrin are effective in promoting osteogenecity and biomechanical stiffness.
| Original language | English |
|---|---|
| Pages (from-to) | 52-56 |
| Number of pages | 5 |
| Journal | Tissue Engineering and Regenerative Medicine |
| Volume | 6 |
| Issue number | 1-3 |
| State | Published - Mar 2009 |
Keywords
- Biomechanical properties
- Fibrin
- Osteoblast