Abstract
Aims: Despite the adequate therapy, there is a subset of patients with early stage oral squamous cell carcinoma, such as tongue cancer, who may have unpredictably poor prognosis. In such cases, it is very helpful to identify novel biological markers which reflect more accurately growth rate, and metastatic potential of each tumors. It is well known that microvessel density (MVD) and p53 gene mutation correlates significantly with tumor behavior in some types of cancer, but some studies have reported a lack of relationship between MVD, p53 gene mutation and tumor behavior in oral cancers. We evaluated the avaliablity of them for deciding the therapeutic plans of the tumor. Methods: Twenty-five tumor specimens of oral squamous cell carcinoma were analyzed microvessel density by immunohistochemical staining with CD-31 monoclonal antibody and exaimed the p53 mutation in exon 5 through 8 by PCR-SSCP and sequencing analysis to identify putative associations between p53 gene mutation and microvessel density (MVD). Results: Seven of the 25 patients had mutation in exon 5 to 8 and all the mutations were missense mutation. The mean of MVD in the mutant group was 13.3±2.80 and that of MVD in wild type group was 18.6±1.16. An inverse relationship was seen between MVD and p53 mutation (P = 0.047). The p53 gene mutation was frequently found in exon 5. Conclusions: In conclusion, MVD and p53 gene mutation were not associated with stage, cervical metastasis and recurrence of the oral cancer. Angiogenesis of oral squamous cell carcinoma might not be regulated by p53 only or regulated by other factors.
Original language | English |
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Pages (from-to) | S58 |
Journal | Oral Oncology |
Volume | 37 |
Issue number | SUPPL. 1 |
State | Published - 2001 |