Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: Comparison with currently available methods

Hyun Sop Choe; Dong Sup Lee; Seung Ju Lee; Sung Hoo Hong; Dong Choon Park; Mi Kyung Lee; Tae Hyoung Kim; Yong Hyun Cho

doi:10.1016/j.ijid.2013.07.011

Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: Comparison with currently available methods

Hyun Sop Choe, Dong Sup Lee, Seung Ju Lee, Sung Hoo Hong, Dong Choon Park, Mi Kyung Lee, Tae Hyoung Kim, Yong Hyun Cho

Research output: Contribution to journal › Article › peer-review

90 Scopus citations

Abstract

Objectives: The real-time PCR assay is the most sensitive test for screening and diagnosing sexually transmitted infections (STIs) and has made diagnosing these infections easier for clinicians. The aim of this study was to investigate the reliability, accuracy, and usefulness of the real-time multiplex PCR assay for the detection of seven sexually transmitted microorganisms in clinical samples. Methods: A total of 897 specimens from 365 symptomatic patients and 532 asymptomatic volunteers were collected over a 10-month period. A total of 696 subjects provided 50ml of first-voided urine as samples, and 201 female patients provided endocervical swab specimens. Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum were tested for using five diagnostic methods: multiplex real-time PCR (Anyplex™ II), multiplex PCR (Seeplex^®), strand displacement amplification (SDA, BD ProbeTec™ ET), PCR (AmpliSens^®), and a commercially available Mycoplasma IST 2 Kit. Results: Multiplex real-time PCR (Anyplex™ II) showed outstanding results in all fields, particularly sensitivity and specificity, compared with other diagnostic tools. This method yielded 100% sensitivity and high specificity for the detection of C. trachomatis, N. gonorrhoeae, T. vaginalis, M. genitalium, and M. hominis. It was also useful for discriminating between U. urealyticum and U. parvum. Conclusions: Multiplex real-time PCR was found to be an equivalent or superior modality for the diagnosis of STIs. It could be a cost-effective and rapid diagnostic tool for the simultaneous detection of multiple STI microorganisms.

Original language	English
Pages (from-to)	e1134-e1140
Journal	International Journal of Infectious Diseases
Volume	17
Issue number	12
DOIs	https://doi.org/10.1016/j.ijid.2013.07.011
State	Published - Dec 2013

Bibliographical note

Funding Information:
This study is an investigator-initiated trial supported by Seegene Inc., Seoul, South Korea. This funding source had no involvement in the study design, conduct, analysis, or publication. We declare that we have no financial conflicts of interest. The authors would like to thank Eun-Sil Jang for assistance with data collection.

Keywords

Bacterial and parasite infection
Diagnosis
Multiplex real-time PCR
Sexually transmitted infection

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijid.2013.07.011

Cite this

Choe, H. S., Lee, D. S., Lee, S. J., Hong, S. H., Park, D. C., Lee, M. K., Kim, T. H., & Cho, Y. H. (2013). Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: Comparison with currently available methods. International Journal of Infectious Diseases, 17(12), e1134-e1140. https://doi.org/10.1016/j.ijid.2013.07.011

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abstract = "Objectives: The real-time PCR assay is the most sensitive test for screening and diagnosing sexually transmitted infections (STIs) and has made diagnosing these infections easier for clinicians. The aim of this study was to investigate the reliability, accuracy, and usefulness of the real-time multiplex PCR assay for the detection of seven sexually transmitted microorganisms in clinical samples. Methods: A total of 897 specimens from 365 symptomatic patients and 532 asymptomatic volunteers were collected over a 10-month period. A total of 696 subjects provided 50ml of first-voided urine as samples, and 201 female patients provided endocervical swab specimens. Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum were tested for using five diagnostic methods: multiplex real-time PCR (Anyplex{\texttrademark} II), multiplex PCR (Seeplex{\textregistered}), strand displacement amplification (SDA, BD ProbeTec{\texttrademark} ET), PCR (AmpliSens{\textregistered}), and a commercially available Mycoplasma IST 2 Kit. Results: Multiplex real-time PCR (Anyplex{\texttrademark} II) showed outstanding results in all fields, particularly sensitivity and specificity, compared with other diagnostic tools. This method yielded 100% sensitivity and high specificity for the detection of C. trachomatis, N. gonorrhoeae, T. vaginalis, M. genitalium, and M. hominis. It was also useful for discriminating between U. urealyticum and U. parvum. Conclusions: Multiplex real-time PCR was found to be an equivalent or superior modality for the diagnosis of STIs. It could be a cost-effective and rapid diagnostic tool for the simultaneous detection of multiple STI microorganisms.",

keywords = "Bacterial and parasite infection, Diagnosis, Multiplex real-time PCR, Sexually transmitted infection",

author = "Choe, {Hyun Sop} and Lee, {Dong Sup} and Lee, {Seung Ju} and Hong, {Sung Hoo} and Park, {Dong Choon} and Lee, {Mi Kyung} and Kim, {Tae Hyoung} and Cho, {Yong Hyun}",

note = "Funding Information: This study is an investigator-initiated trial supported by Seegene Inc., Seoul, South Korea. This funding source had no involvement in the study design, conduct, analysis, or publication. We declare that we have no financial conflicts of interest. The authors would like to thank Eun-Sil Jang for assistance with data collection. ",

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doi = "10.1016/j.ijid.2013.07.011",

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Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: Comparison with currently available methods. / Choe, Hyun Sop; Lee, Dong Sup ; Lee, Seung Ju et al.
In: International Journal of Infectious Diseases, Vol. 17, No. 12, 12.2013, p. e1134-e1140.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections

T2 - Comparison with currently available methods

AU - Choe, Hyun Sop

AU - Lee, Dong Sup

AU - Lee, Seung Ju

AU - Hong, Sung Hoo

AU - Park, Dong Choon

AU - Lee, Mi Kyung

AU - Kim, Tae Hyoung

AU - Cho, Yong Hyun

N1 - Funding Information: This study is an investigator-initiated trial supported by Seegene Inc., Seoul, South Korea. This funding source had no involvement in the study design, conduct, analysis, or publication. We declare that we have no financial conflicts of interest. The authors would like to thank Eun-Sil Jang for assistance with data collection.

PY - 2013/12

Y1 - 2013/12

N2 - Objectives: The real-time PCR assay is the most sensitive test for screening and diagnosing sexually transmitted infections (STIs) and has made diagnosing these infections easier for clinicians. The aim of this study was to investigate the reliability, accuracy, and usefulness of the real-time multiplex PCR assay for the detection of seven sexually transmitted microorganisms in clinical samples. Methods: A total of 897 specimens from 365 symptomatic patients and 532 asymptomatic volunteers were collected over a 10-month period. A total of 696 subjects provided 50ml of first-voided urine as samples, and 201 female patients provided endocervical swab specimens. Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum were tested for using five diagnostic methods: multiplex real-time PCR (Anyplex™ II), multiplex PCR (Seeplex®), strand displacement amplification (SDA, BD ProbeTec™ ET), PCR (AmpliSens®), and a commercially available Mycoplasma IST 2 Kit. Results: Multiplex real-time PCR (Anyplex™ II) showed outstanding results in all fields, particularly sensitivity and specificity, compared with other diagnostic tools. This method yielded 100% sensitivity and high specificity for the detection of C. trachomatis, N. gonorrhoeae, T. vaginalis, M. genitalium, and M. hominis. It was also useful for discriminating between U. urealyticum and U. parvum. Conclusions: Multiplex real-time PCR was found to be an equivalent or superior modality for the diagnosis of STIs. It could be a cost-effective and rapid diagnostic tool for the simultaneous detection of multiple STI microorganisms.

AB - Objectives: The real-time PCR assay is the most sensitive test for screening and diagnosing sexually transmitted infections (STIs) and has made diagnosing these infections easier for clinicians. The aim of this study was to investigate the reliability, accuracy, and usefulness of the real-time multiplex PCR assay for the detection of seven sexually transmitted microorganisms in clinical samples. Methods: A total of 897 specimens from 365 symptomatic patients and 532 asymptomatic volunteers were collected over a 10-month period. A total of 696 subjects provided 50ml of first-voided urine as samples, and 201 female patients provided endocervical swab specimens. Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum were tested for using five diagnostic methods: multiplex real-time PCR (Anyplex™ II), multiplex PCR (Seeplex®), strand displacement amplification (SDA, BD ProbeTec™ ET), PCR (AmpliSens®), and a commercially available Mycoplasma IST 2 Kit. Results: Multiplex real-time PCR (Anyplex™ II) showed outstanding results in all fields, particularly sensitivity and specificity, compared with other diagnostic tools. This method yielded 100% sensitivity and high specificity for the detection of C. trachomatis, N. gonorrhoeae, T. vaginalis, M. genitalium, and M. hominis. It was also useful for discriminating between U. urealyticum and U. parvum. Conclusions: Multiplex real-time PCR was found to be an equivalent or superior modality for the diagnosis of STIs. It could be a cost-effective and rapid diagnostic tool for the simultaneous detection of multiple STI microorganisms.

KW - Bacterial and parasite infection

KW - Diagnosis

KW - Multiplex real-time PCR

KW - Sexually transmitted infection

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DO - 10.1016/j.ijid.2013.07.011

M3 - Article

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AN - SCOPUS:84888440180

SN - 1201-9712

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ER -

Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: Comparison with currently available methods

Abstract

Bibliographical note

Keywords

UN SDGs

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