Phenotypic and genomic characterization of AmpC-producing Klebsiella pneumoniae from Korea

Mattia Palmieri; Stephane Schicklin; Andreu Coello Pelegrin; Sonia Chatellier; Christine Franceschi; Caroline Mirande; Yeon Joon Park; Alex Van Belkum

doi:10.3343/alm.2018.38.4.367

Phenotypic and genomic characterization of AmpC-producing Klebsiella pneumoniae from Korea

Mattia Palmieri, Stephane Schicklin, Andreu Coello Pelegrin, Sonia Chatellier, Christine Franceschi, Caroline Mirande, Yeon Joon Park, Alex Van Belkum

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The prevalence of multidrug-resistant gram-negative bacteria has continuously increased over the past few years; bacterial strains producing AmpC β-lactamases and/or extended-spectrum β-lactamases (ESBLs) are of particular concern. We combined high-resolution whole genome sequencing and phenotypic data to elucidate the mechanisms of resistance to cephamycin and β-lactamase in Korean Klebsiella pneumoniae strains, in which no AmpC-encoding genes were detected by PCR. We identified several genes that alone or in combination can potentially explain the resistance phenotype. We showed that different mechanisms could explain the resistance phenotype, emphasizing the limitations of the PCR and the importance of distinguishing closely-related gene variants.

Original language	English
Pages (from-to)	367-370
Number of pages	4
Journal	Annals of Laboratory Medicine
Volume	38
Issue number	4
DOIs	https://doi.org/10.3343/alm.2018.38.4.367
State	Published - 2018

Bibliographical note

Funding Information:
The work performed by Mattia Palmieri and Andreu Coello Pel-egrin was funded by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No 675412 (New Diagnostics for Infectious Diseases [ND4ID]).

Publisher Copyright:
© Korean Society for Laboratory Medicine.

Keywords

AmpC
Antibiotic resistance
Klebsiella pneumoniae
Korea
Whole Genome Sequencing

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10.3343/alm.2018.38.4.367

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title = "Phenotypic and genomic characterization of AmpC-producing Klebsiella pneumoniae from Korea",

abstract = "The prevalence of multidrug-resistant gram-negative bacteria has continuously increased over the past few years; bacterial strains producing AmpC β-lactamases and/or extended-spectrum β-lactamases (ESBLs) are of particular concern. We combined high-resolution whole genome sequencing and phenotypic data to elucidate the mechanisms of resistance to cephamycin and β-lactamase in Korean Klebsiella pneumoniae strains, in which no AmpC-encoding genes were detected by PCR. We identified several genes that alone or in combination can potentially explain the resistance phenotype. We showed that different mechanisms could explain the resistance phenotype, emphasizing the limitations of the PCR and the importance of distinguishing closely-related gene variants.",

keywords = "AmpC, Antibiotic resistance, Klebsiella pneumoniae, Korea, Whole Genome Sequencing",

author = "Mattia Palmieri and Stephane Schicklin and Pelegrin, \{Andreu Coello\} and Sonia Chatellier and Christine Franceschi and Caroline Mirande and Park, \{Yeon Joon\} and \{Van Belkum\}, Alex",

note = "Funding Information: The work performed by Mattia Palmieri and Andreu Coello Pel-egrin was funded by the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie Grant Agreement No 675412 (New Diagnostics for Infectious Diseases [ND4ID]). Publisher Copyright: {\textcopyright} Korean Society for Laboratory Medicine.",

year = "2018",

doi = "10.3343/alm.2018.38.4.367",

language = "English",

volume = "38",

pages = "367--370",

journal = "Annals of Laboratory Medicine",

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TY - JOUR

T1 - Phenotypic and genomic characterization of AmpC-producing Klebsiella pneumoniae from Korea

AU - Palmieri, Mattia

AU - Schicklin, Stephane

AU - Pelegrin, Andreu Coello

AU - Chatellier, Sonia

AU - Franceschi, Christine

AU - Mirande, Caroline

AU - Park, Yeon Joon

AU - Van Belkum, Alex

N1 - Funding Information: The work performed by Mattia Palmieri and Andreu Coello Pel-egrin was funded by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No 675412 (New Diagnostics for Infectious Diseases [ND4ID]). Publisher Copyright: © Korean Society for Laboratory Medicine.

PY - 2018

Y1 - 2018

N2 - The prevalence of multidrug-resistant gram-negative bacteria has continuously increased over the past few years; bacterial strains producing AmpC β-lactamases and/or extended-spectrum β-lactamases (ESBLs) are of particular concern. We combined high-resolution whole genome sequencing and phenotypic data to elucidate the mechanisms of resistance to cephamycin and β-lactamase in Korean Klebsiella pneumoniae strains, in which no AmpC-encoding genes were detected by PCR. We identified several genes that alone or in combination can potentially explain the resistance phenotype. We showed that different mechanisms could explain the resistance phenotype, emphasizing the limitations of the PCR and the importance of distinguishing closely-related gene variants.

AB - The prevalence of multidrug-resistant gram-negative bacteria has continuously increased over the past few years; bacterial strains producing AmpC β-lactamases and/or extended-spectrum β-lactamases (ESBLs) are of particular concern. We combined high-resolution whole genome sequencing and phenotypic data to elucidate the mechanisms of resistance to cephamycin and β-lactamase in Korean Klebsiella pneumoniae strains, in which no AmpC-encoding genes were detected by PCR. We identified several genes that alone or in combination can potentially explain the resistance phenotype. We showed that different mechanisms could explain the resistance phenotype, emphasizing the limitations of the PCR and the importance of distinguishing closely-related gene variants.

KW - AmpC

KW - Antibiotic resistance

KW - Klebsiella pneumoniae

KW - Korea

KW - Whole Genome Sequencing

UR - https://www.scopus.com/pages/publications/85045224209

U2 - 10.3343/alm.2018.38.4.367

DO - 10.3343/alm.2018.38.4.367

M3 - Article

C2 - 29611388

AN - SCOPUS:85045224209

SN - 2234-3806

VL - 38

SP - 367

EP - 370

JO - Annals of Laboratory Medicine

JF - Annals of Laboratory Medicine

IS - 4

ER -

Phenotypic and genomic characterization of AmpC-producing Klebsiella pneumoniae from Korea

Abstract

Bibliographical note

Keywords

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