Prevalence and characteristics of aac(6′)-Ib-cr in AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens: a multicenter study from Korea

Soo Young Kim; Yeon Joon Park; Jin Kyung Yu; Yeong Sic Kim; Kyungja Han

doi:10.1016/j.diagmicrobio.2008.11.016

Prevalence and characteristics of aac(6′)-Ib-cr in AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens: a multicenter study from Korea

Soo Young Kim
, Yeon Joon Park
, Jin Kyung Yu
, Yeong Sic Kim
, Kyungja Han

Department of Laboratory Medicine

The Catholic University of Korea

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

We investigated the prevalence of aac(6′)-Ib-cr and its association with other resistance genes in AmpC-producing Enterobacteriaceae without any selection criteria. A total of 479 clinical isolates of Enterobacter cloacae (179), Citrobacter freundii (134), and Serratia marcescens (166) from 12 laboratories between March and July 2005 were examined. We performed polymerase chain reaction for aac(6′)-Ib, bla_OXA-1, ISEcp1, and class 1 integron. The aac(6′)-Ib-cr was further identified by digestion with BstF5I and sequencing. The aac(6′)-Ib was detected in 110 (23%) of 479 isolates, and 15 isolates (3.1%) were cr variants (8 E. cloacae, 5 C. freundii, and 2 S. marcescens). The aac(6′)-Ib-cr was significantly associated with various resistance genes (bla_OXA-1, qnrS, qnrA, bla_CTX-M-3, and bla_CTX-M-14), mobile elements (ISEcp1, ISCR1, and class 1 integron), and quinolone resistance. Eleven of 15 aac(6′)-Ib-cr producers coharbored qnr genes. Although aac(6′)-Ib-cr was uncommon in Korean AmpC producers, its association with various resistance genes and mobile elements would facilitate the dissemination of this variant.

Original language	English
Pages (from-to)	314-318
Number of pages	5
Journal	Diagnostic Microbiology and Infectious Disease
Volume	63
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2008.11.016
State	Published - Mar 2009

Bibliographical note

Funding Information:
The authors thank all the contributing laboratories that provided isolates for this study. We are indebted to Jung Jun Park, Kang Gyun Park, and Dong Wha Han for valuable technical assistance. This work was supported by a grant from Catholic Medical Center Research Foundation in 2007 and in part from the Korea Food and Drug Administration (07052-antibiotics 174).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Quinolone
Resistance
aac(6′)-Ib-cr
bla
qnr

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10.1016/j.diagmicrobio.2008.11.016

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@article{f33fd2f698b04089bce62346a750e328,

title = "Prevalence and characteristics of aac(6′)-Ib-cr in AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens: a multicenter study from Korea",

abstract = "We investigated the prevalence of aac(6′)-Ib-cr and its association with other resistance genes in AmpC-producing Enterobacteriaceae without any selection criteria. A total of 479 clinical isolates of Enterobacter cloacae (179), Citrobacter freundii (134), and Serratia marcescens (166) from 12 laboratories between March and July 2005 were examined. We performed polymerase chain reaction for aac(6′)-Ib, blaOXA-1, ISEcp1, and class 1 integron. The aac(6′)-Ib-cr was further identified by digestion with BstF5I and sequencing. The aac(6′)-Ib was detected in 110 (23\%) of 479 isolates, and 15 isolates (3.1\%) were cr variants (8 E. cloacae, 5 C. freundii, and 2 S. marcescens). The aac(6′)-Ib-cr was significantly associated with various resistance genes (blaOXA-1, qnrS, qnrA, blaCTX-M-3, and blaCTX-M-14), mobile elements (ISEcp1, ISCR1, and class 1 integron), and quinolone resistance. Eleven of 15 aac(6′)-Ib-cr producers coharbored qnr genes. Although aac(6′)-Ib-cr was uncommon in Korean AmpC producers, its association with various resistance genes and mobile elements would facilitate the dissemination of this variant.",

keywords = "Quinolone, Resistance, aac(6′)-Ib-cr, bla, qnr",

author = "Kim, \{Soo Young\} and Park, \{Yeon Joon\} and Yu, \{Jin Kyung\} and Kim, \{Yeong Sic\} and Kyungja Han",

note = "Funding Information: The authors thank all the contributing laboratories that provided isolates for this study. We are indebted to Jung Jun Park, Kang Gyun Park, and Dong Wha Han for valuable technical assistance. This work was supported by a grant from Catholic Medical Center Research Foundation in 2007 and in part from the Korea Food and Drug Administration (07052-antibiotics 174).",

year = "2009",

month = mar,

doi = "10.1016/j.diagmicrobio.2008.11.016",

language = "English",

volume = "63",

pages = "314--318",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "3",

}

Prevalence and characteristics of aac(6′)-Ib-cr in AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens: a multicenter study from Korea. / Kim, Soo Young; Park, Yeon Joon; Yu, Jin Kyung et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 63, No. 3, 03.2009, p. 314-318.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Prevalence and characteristics of aac(6′)-Ib-cr in AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens

T2 - a multicenter study from Korea

AU - Kim, Soo Young

AU - Park, Yeon Joon

AU - Yu, Jin Kyung

AU - Kim, Yeong Sic

AU - Han, Kyungja

N1 - Funding Information: The authors thank all the contributing laboratories that provided isolates for this study. We are indebted to Jung Jun Park, Kang Gyun Park, and Dong Wha Han for valuable technical assistance. This work was supported by a grant from Catholic Medical Center Research Foundation in 2007 and in part from the Korea Food and Drug Administration (07052-antibiotics 174).

PY - 2009/3

Y1 - 2009/3

N2 - We investigated the prevalence of aac(6′)-Ib-cr and its association with other resistance genes in AmpC-producing Enterobacteriaceae without any selection criteria. A total of 479 clinical isolates of Enterobacter cloacae (179), Citrobacter freundii (134), and Serratia marcescens (166) from 12 laboratories between March and July 2005 were examined. We performed polymerase chain reaction for aac(6′)-Ib, blaOXA-1, ISEcp1, and class 1 integron. The aac(6′)-Ib-cr was further identified by digestion with BstF5I and sequencing. The aac(6′)-Ib was detected in 110 (23%) of 479 isolates, and 15 isolates (3.1%) were cr variants (8 E. cloacae, 5 C. freundii, and 2 S. marcescens). The aac(6′)-Ib-cr was significantly associated with various resistance genes (blaOXA-1, qnrS, qnrA, blaCTX-M-3, and blaCTX-M-14), mobile elements (ISEcp1, ISCR1, and class 1 integron), and quinolone resistance. Eleven of 15 aac(6′)-Ib-cr producers coharbored qnr genes. Although aac(6′)-Ib-cr was uncommon in Korean AmpC producers, its association with various resistance genes and mobile elements would facilitate the dissemination of this variant.

AB - We investigated the prevalence of aac(6′)-Ib-cr and its association with other resistance genes in AmpC-producing Enterobacteriaceae without any selection criteria. A total of 479 clinical isolates of Enterobacter cloacae (179), Citrobacter freundii (134), and Serratia marcescens (166) from 12 laboratories between March and July 2005 were examined. We performed polymerase chain reaction for aac(6′)-Ib, blaOXA-1, ISEcp1, and class 1 integron. The aac(6′)-Ib-cr was further identified by digestion with BstF5I and sequencing. The aac(6′)-Ib was detected in 110 (23%) of 479 isolates, and 15 isolates (3.1%) were cr variants (8 E. cloacae, 5 C. freundii, and 2 S. marcescens). The aac(6′)-Ib-cr was significantly associated with various resistance genes (blaOXA-1, qnrS, qnrA, blaCTX-M-3, and blaCTX-M-14), mobile elements (ISEcp1, ISCR1, and class 1 integron), and quinolone resistance. Eleven of 15 aac(6′)-Ib-cr producers coharbored qnr genes. Although aac(6′)-Ib-cr was uncommon in Korean AmpC producers, its association with various resistance genes and mobile elements would facilitate the dissemination of this variant.

KW - Quinolone

KW - Resistance

KW - aac(6′)-Ib-cr

KW - bla

KW - qnr

UR - https://www.scopus.com/pages/publications/59649092179

U2 - 10.1016/j.diagmicrobio.2008.11.016

DO - 10.1016/j.diagmicrobio.2008.11.016

M3 - Article

C2 - 19216942

AN - SCOPUS:59649092179

SN - 0732-8893

VL - 63

SP - 314

EP - 318

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

ER -

Prevalence and characteristics of aac(6′)-Ib-cr in AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens: a multicenter study from Korea

Abstract

Bibliographical note

UN SDGs

Keywords

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