Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)

Ji Sun Lee; Andriy Synytsya; Hyun Bok Kim; Doo Jin Choi; Seul Lee; Jisun Lee; Woo Jung Kim; Seongjae Jang; Yong Il Park

doi:10.1016/j.intimp.2013.09.019

Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)

Ji Sun Lee, Andriy Synytsya, Hyun Bok Kim, Doo Jin Choi, Seul Lee, Jisun Lee, Woo Jung Kim, Seongjae Jang, Yong Il Park

Department of Medical and Biological Sciences

Research output: Contribution to journal › Article › peer-review

141 Scopus citations

Abstract

A water-soluble polysaccharide (JS-MP-1) was isolated and purified from the Korean mulberry fruits Oddi (Morus alba L.) by crushing the fresh fruits then performing ethanol precipitation and DEAE-cellulose ion exchange chromatography. The neutral monosaccharide composition of the purified JS-MP-1 was determined to be composed mainly of galactose (37.6%, in mole percent), arabinose (36.3%), and rhamnose (18.4%), while other major sugars such as glucose, xylose, mannose, and fucose were present as minor components. HPLC analysis revealed that JS-MP-1 contains both galacturonic acid (GalA) and glucuronic acid (GlcA) at approximately 4:1 in mole percent. Monosaccharide composition, Fourier-transform infrared (FTIR) analysis, biochemical analysis, and elemental analysis suggested that JS-MP-1 is an acidic heteropolysaccharide, most likely a rhamnoarabinogalacturonan type plant pectic polysaccharide, with an apparent molecular mass of 1600 kDa containing no, or if any, negligible level of sulfate esters and proteins. Enzyme-Linked Immunosorbent Assay and RT-PCR analysis demonstrated that JS-MP-1 significantly stimulates murine RAW264.7 macrophage cells to release chemokines (RANTES and MIP-1α) and proinflammatory cytokines (TNF-α and IL-6) and to induce the expression of iNOS and COX-2, which are responsible for the production of NO and prostaglandin PGE2, respectively. These results suggest that the mulberry fruit-derived polysaccharide JS-MP-1 can act as a potent immunomodulator, and these observations may support the applicability of this polysaccharide as an immunotherapeutic adjuvant or the water extracts of the mulberry fruit as a beneficial health food.

Original language	English
Pages (from-to)	858-866
Number of pages	9
Journal	International Immunopharmacology
Volume	17
Issue number	3
DOIs	https://doi.org/10.1016/j.intimp.2013.09.019
State	Published - 2013

Bibliographical note

Funding Information:
This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ0071862011 ), Rural Development Administration, Republic of Korea , by the Gyeonggi-do Regional Research Centre (GRRC) program at the Catholic University of Korea, by the Research Fund 2012 of the Catholic University of Korea and partly by the Ministry of Education of the Czech Republic (Project CEZ: MSM6046137305 ), the Slovak Scientific Grant Agency (VEGA No. 2/0017/11 ), for which the authors are thankful.

Keywords

Immunomodulation
Macrophages
Mulberry fruit
Polysaccharide

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10.1016/j.intimp.2013.09.019

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@article{da3e059f7c6c4d978814ad1c6f35c146,

title = "Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)",

abstract = "A water-soluble polysaccharide (JS-MP-1) was isolated and purified from the Korean mulberry fruits Oddi (Morus alba L.) by crushing the fresh fruits then performing ethanol precipitation and DEAE-cellulose ion exchange chromatography. The neutral monosaccharide composition of the purified JS-MP-1 was determined to be composed mainly of galactose (37.6%, in mole percent), arabinose (36.3%), and rhamnose (18.4%), while other major sugars such as glucose, xylose, mannose, and fucose were present as minor components. HPLC analysis revealed that JS-MP-1 contains both galacturonic acid (GalA) and glucuronic acid (GlcA) at approximately 4:1 in mole percent. Monosaccharide composition, Fourier-transform infrared (FTIR) analysis, biochemical analysis, and elemental analysis suggested that JS-MP-1 is an acidic heteropolysaccharide, most likely a rhamnoarabinogalacturonan type plant pectic polysaccharide, with an apparent molecular mass of 1600 kDa containing no, or if any, negligible level of sulfate esters and proteins. Enzyme-Linked Immunosorbent Assay and RT-PCR analysis demonstrated that JS-MP-1 significantly stimulates murine RAW264.7 macrophage cells to release chemokines (RANTES and MIP-1α) and proinflammatory cytokines (TNF-α and IL-6) and to induce the expression of iNOS and COX-2, which are responsible for the production of NO and prostaglandin PGE2, respectively. These results suggest that the mulberry fruit-derived polysaccharide JS-MP-1 can act as a potent immunomodulator, and these observations may support the applicability of this polysaccharide as an immunotherapeutic adjuvant or the water extracts of the mulberry fruit as a beneficial health food.",

keywords = "Immunomodulation, Macrophages, Mulberry fruit, Polysaccharide",

author = "Lee, {Ji Sun} and Andriy Synytsya and Kim, {Hyun Bok} and Choi, {Doo Jin} and Seul Lee and Jisun Lee and Kim, {Woo Jung} and Seongjae Jang and Park, {Yong Il}",

note = "Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ0071862011 ), Rural Development Administration, Republic of Korea , by the Gyeonggi-do Regional Research Centre (GRRC) program at the Catholic University of Korea, by the Research Fund 2012 of the Catholic University of Korea and partly by the Ministry of Education of the Czech Republic (Project CEZ: MSM6046137305 ), the Slovak Scientific Grant Agency (VEGA No. 2/0017/11 ), for which the authors are thankful.",

year = "2013",

doi = "10.1016/j.intimp.2013.09.019",

language = "English",

volume = "17",

pages = "858--866",

journal = "International Immunopharmacology",

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AU - Lee, Ji Sun

AU - Synytsya, Andriy

AU - Kim, Hyun Bok

AU - Choi, Doo Jin

AU - Lee, Seul

AU - Lee, Jisun

AU - Kim, Woo Jung

AU - Jang, Seongjae

AU - Park, Yong Il

N1 - Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ0071862011 ), Rural Development Administration, Republic of Korea , by the Gyeonggi-do Regional Research Centre (GRRC) program at the Catholic University of Korea, by the Research Fund 2012 of the Catholic University of Korea and partly by the Ministry of Education of the Czech Republic (Project CEZ: MSM6046137305 ), the Slovak Scientific Grant Agency (VEGA No. 2/0017/11 ), for which the authors are thankful.

PY - 2013

Y1 - 2013

N2 - A water-soluble polysaccharide (JS-MP-1) was isolated and purified from the Korean mulberry fruits Oddi (Morus alba L.) by crushing the fresh fruits then performing ethanol precipitation and DEAE-cellulose ion exchange chromatography. The neutral monosaccharide composition of the purified JS-MP-1 was determined to be composed mainly of galactose (37.6%, in mole percent), arabinose (36.3%), and rhamnose (18.4%), while other major sugars such as glucose, xylose, mannose, and fucose were present as minor components. HPLC analysis revealed that JS-MP-1 contains both galacturonic acid (GalA) and glucuronic acid (GlcA) at approximately 4:1 in mole percent. Monosaccharide composition, Fourier-transform infrared (FTIR) analysis, biochemical analysis, and elemental analysis suggested that JS-MP-1 is an acidic heteropolysaccharide, most likely a rhamnoarabinogalacturonan type plant pectic polysaccharide, with an apparent molecular mass of 1600 kDa containing no, or if any, negligible level of sulfate esters and proteins. Enzyme-Linked Immunosorbent Assay and RT-PCR analysis demonstrated that JS-MP-1 significantly stimulates murine RAW264.7 macrophage cells to release chemokines (RANTES and MIP-1α) and proinflammatory cytokines (TNF-α and IL-6) and to induce the expression of iNOS and COX-2, which are responsible for the production of NO and prostaglandin PGE2, respectively. These results suggest that the mulberry fruit-derived polysaccharide JS-MP-1 can act as a potent immunomodulator, and these observations may support the applicability of this polysaccharide as an immunotherapeutic adjuvant or the water extracts of the mulberry fruit as a beneficial health food.

AB - A water-soluble polysaccharide (JS-MP-1) was isolated and purified from the Korean mulberry fruits Oddi (Morus alba L.) by crushing the fresh fruits then performing ethanol precipitation and DEAE-cellulose ion exchange chromatography. The neutral monosaccharide composition of the purified JS-MP-1 was determined to be composed mainly of galactose (37.6%, in mole percent), arabinose (36.3%), and rhamnose (18.4%), while other major sugars such as glucose, xylose, mannose, and fucose were present as minor components. HPLC analysis revealed that JS-MP-1 contains both galacturonic acid (GalA) and glucuronic acid (GlcA) at approximately 4:1 in mole percent. Monosaccharide composition, Fourier-transform infrared (FTIR) analysis, biochemical analysis, and elemental analysis suggested that JS-MP-1 is an acidic heteropolysaccharide, most likely a rhamnoarabinogalacturonan type plant pectic polysaccharide, with an apparent molecular mass of 1600 kDa containing no, or if any, negligible level of sulfate esters and proteins. Enzyme-Linked Immunosorbent Assay and RT-PCR analysis demonstrated that JS-MP-1 significantly stimulates murine RAW264.7 macrophage cells to release chemokines (RANTES and MIP-1α) and proinflammatory cytokines (TNF-α and IL-6) and to induce the expression of iNOS and COX-2, which are responsible for the production of NO and prostaglandin PGE2, respectively. These results suggest that the mulberry fruit-derived polysaccharide JS-MP-1 can act as a potent immunomodulator, and these observations may support the applicability of this polysaccharide as an immunotherapeutic adjuvant or the water extracts of the mulberry fruit as a beneficial health food.

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KW - Macrophages

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ER -

Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)

Abstract

Bibliographical note

Keywords

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