Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens

Ye Seul Na; Woo Jung Kim; Sung Min Kim; Jae Kweon Park; Sae Mi Lee; Sung Oog Kim; Andriy Synytsya; Yong Il Park

doi:10.1016/j.intimp.2009.12.011

Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens

Ye Seul Na, Woo Jung Kim, Sung Min Kim, Jae Kweon Park, Sae Mi Lee, Sung Oog Kim, Andriy Synytsya, Yong Il Park

Department of Medical and Biological Sciences

Research output: Contribution to journal › Article › peer-review

127 Scopus citations

Abstract

A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the monosaccharide composition of the SPS-CF was determined to be mannose (55.4% in mole percentage), galactose (25.3%), glucuronic acid (16.3%), and arabinose (0.8%). Fourier-transform infrared and elemental analysis indicated that the purified SPS-CF is a sulfated polysaccharide containing significant amount of sulfate esters (5.7% in mass). Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. These results suggest that the sulfated SPS-CF isolated from C. fulvescens has potent immunostimulating activity.

Original language	English
Pages (from-to)	364-370
Number of pages	7
Journal	International Immunopharmacology
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/j.intimp.2009.12.011
State	Published - Mar 2010

Bibliographical note

Funding Information:
This study was supported by a grant from the Gyeonggi-do GRRC program and partly by the Research Fund, 2009 of The Catholic University of Korea .

Keywords

Capsosiphon fulvescens
Immunostimulation
Macrophages
Sulfated polysaccharide

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10.1016/j.intimp.2009.12.011

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title = "Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens",

abstract = "A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the monosaccharide composition of the SPS-CF was determined to be mannose (55.4% in mole percentage), galactose (25.3%), glucuronic acid (16.3%), and arabinose (0.8%). Fourier-transform infrared and elemental analysis indicated that the purified SPS-CF is a sulfated polysaccharide containing significant amount of sulfate esters (5.7% in mass). Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. These results suggest that the sulfated SPS-CF isolated from C. fulvescens has potent immunostimulating activity.",

keywords = "Capsosiphon fulvescens, Immunostimulation, Macrophages, Sulfated polysaccharide",

author = "Na, {Ye Seul} and Kim, {Woo Jung} and Kim, {Sung Min} and Park, {Jae Kweon} and Lee, {Sae Mi} and Kim, {Sung Oog} and Andriy Synytsya and Park, {Yong Il}",

note = "Funding Information: This study was supported by a grant from the Gyeonggi-do GRRC program and partly by the Research Fund, 2009 of The Catholic University of Korea .",

year = "2010",

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doi = "10.1016/j.intimp.2009.12.011",

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T1 - Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens

AU - Na, Ye Seul

AU - Kim, Woo Jung

AU - Kim, Sung Min

AU - Park, Jae Kweon

AU - Lee, Sae Mi

AU - Kim, Sung Oog

AU - Synytsya, Andriy

AU - Park, Yong Il

N1 - Funding Information: This study was supported by a grant from the Gyeonggi-do GRRC program and partly by the Research Fund, 2009 of The Catholic University of Korea .

PY - 2010/3

Y1 - 2010/3

N2 - A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the monosaccharide composition of the SPS-CF was determined to be mannose (55.4% in mole percentage), galactose (25.3%), glucuronic acid (16.3%), and arabinose (0.8%). Fourier-transform infrared and elemental analysis indicated that the purified SPS-CF is a sulfated polysaccharide containing significant amount of sulfate esters (5.7% in mass). Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. These results suggest that the sulfated SPS-CF isolated from C. fulvescens has potent immunostimulating activity.

AB - A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the monosaccharide composition of the SPS-CF was determined to be mannose (55.4% in mole percentage), galactose (25.3%), glucuronic acid (16.3%), and arabinose (0.8%). Fourier-transform infrared and elemental analysis indicated that the purified SPS-CF is a sulfated polysaccharide containing significant amount of sulfate esters (5.7% in mass). Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. These results suggest that the sulfated SPS-CF isolated from C. fulvescens has potent immunostimulating activity.

KW - Capsosiphon fulvescens

KW - Immunostimulation

KW - Macrophages

KW - Sulfated polysaccharide

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SN - 1567-5769

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JF - International Immunopharmacology

IS - 3

ER -

Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens

Abstract

Bibliographical note

Keywords

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