Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens

Ye Seul Na; Woo Jung Kim; Sung Min Kim; Jae Kweon Park; Sae Mi Lee; Sung Oog Kim; Andriy Synytsya; Yong Il Park

doi:10.1016/j.intimp.2009.12.011

Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens

Ye Seul Na
, Woo Jung Kim
, Sung Min Kim
, Jae Kweon Park
, Sae Mi Lee
, Sung Oog Kim
, Andriy Synytsya
, Yong Il Park

Department of Medical and Biological Sciences

The Catholic University of Korea
University of Chemistry and Technology, Prague

Research output: Contribution to journal › Article › peer-review

136 Scopus citations

Abstract

A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the monosaccharide composition of the SPS-CF was determined to be mannose (55.4% in mole percentage), galactose (25.3%), glucuronic acid (16.3%), and arabinose (0.8%). Fourier-transform infrared and elemental analysis indicated that the purified SPS-CF is a sulfated polysaccharide containing significant amount of sulfate esters (5.7% in mass). Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. These results suggest that the sulfated SPS-CF isolated from C. fulvescens has potent immunostimulating activity.

Original language	English
Pages (from-to)	364-370
Number of pages	7
Journal	International Immunopharmacology
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/j.intimp.2009.12.011
State	Published - Mar 2010

Bibliographical note

Funding Information:
This study was supported by a grant from the Gyeonggi-do GRRC program and partly by the Research Fund, 2009 of The Catholic University of Korea .

Keywords

Capsosiphon fulvescens
Immunostimulation
Macrophages
Sulfated polysaccharide

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10.1016/j.intimp.2009.12.011

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title = "Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens",

abstract = "A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the monosaccharide composition of the SPS-CF was determined to be mannose (55.4\% in mole percentage), galactose (25.3\%), glucuronic acid (16.3\%), and arabinose (0.8\%). Fourier-transform infrared and elemental analysis indicated that the purified SPS-CF is a sulfated polysaccharide containing significant amount of sulfate esters (5.7\% in mass). Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. These results suggest that the sulfated SPS-CF isolated from C. fulvescens has potent immunostimulating activity.",

keywords = "Capsosiphon fulvescens, Immunostimulation, Macrophages, Sulfated polysaccharide",

author = "Na, \{Ye Seul\} and Kim, \{Woo Jung\} and Kim, \{Sung Min\} and Park, \{Jae Kweon\} and Lee, \{Sae Mi\} and Kim, \{Sung Oog\} and Andriy Synytsya and Park, \{Yong Il\}",

note = "Funding Information: This study was supported by a grant from the Gyeonggi-do GRRC program and partly by the Research Fund, 2009 of The Catholic University of Korea .",

year = "2010",

month = mar,

doi = "10.1016/j.intimp.2009.12.011",

language = "English",

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T1 - Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens

AU - Na, Ye Seul

AU - Kim, Woo Jung

AU - Kim, Sung Min

AU - Park, Jae Kweon

AU - Lee, Sae Mi

AU - Kim, Sung Oog

AU - Synytsya, Andriy

AU - Park, Yong Il

N1 - Funding Information: This study was supported by a grant from the Gyeonggi-do GRRC program and partly by the Research Fund, 2009 of The Catholic University of Korea .

PY - 2010/3

Y1 - 2010/3

N2 - A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the monosaccharide composition of the SPS-CF was determined to be mannose (55.4% in mole percentage), galactose (25.3%), glucuronic acid (16.3%), and arabinose (0.8%). Fourier-transform infrared and elemental analysis indicated that the purified SPS-CF is a sulfated polysaccharide containing significant amount of sulfate esters (5.7% in mass). Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. These results suggest that the sulfated SPS-CF isolated from C. fulvescens has potent immunostimulating activity.

AB - A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the monosaccharide composition of the SPS-CF was determined to be mannose (55.4% in mole percentage), galactose (25.3%), glucuronic acid (16.3%), and arabinose (0.8%). Fourier-transform infrared and elemental analysis indicated that the purified SPS-CF is a sulfated polysaccharide containing significant amount of sulfate esters (5.7% in mass). Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. These results suggest that the sulfated SPS-CF isolated from C. fulvescens has potent immunostimulating activity.

KW - Capsosiphon fulvescens

KW - Immunostimulation

KW - Macrophages

KW - Sulfated polysaccharide

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M3 - Article

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AN - SCOPUS:76849088569

SN - 1567-5769

VL - 10

SP - 364

EP - 370

JO - International Immunopharmacology

JF - International Immunopharmacology

IS - 3

ER -

Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens

Abstract

Bibliographical note

Keywords

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