Reduced autophagy in 5-fluorouracil resistant colon cancer cells

Cheng Wen Yao; Kyoung Ah Kang; Mei Jing Piao; Yea Seong Ryu; Pattage Madushan Dilhara Jayatissa Fernando; Min Chang Oh; Jeong Eon Park; Kristina Shilnikova; Soo Young Na; Seung Uk Jeong; Sun Jin Boo; Jin Won Hyun

doi:10.4062/biomolther.2016.069

Reduced autophagy in 5-fluorouracil resistant colon cancer cells

Cheng Wen Yao
, Kyoung Ah Kang
, Mei Jing Piao
, Yea Seong Ryu
, Pattage Madushan Dilhara Jayatissa Fernando
, Min Chang Oh
, Jeong Eon Park
, Kristina Shilnikova
, Soo Young Na
, Seung Uk Jeong
, Sun Jin Boo
, Jin Won Hyun

Department of Internal Medicine

Jeju National University

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.

Original language	English
Pages (from-to)	315-320
Number of pages	6
Journal	Biomolecules and Therapeutics
Volume	25
Issue number	3
DOIs	https://doi.org/10.4062/biomolther.2016.069
State	Published - May 2017

Bibliographical note

Publisher Copyright:
© 2017 The Korean Society of Applied Pharmacology.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

5-Fluorouracil
Autophagy
Colon cancer
Reactive oxygen species
SNUC5/5-FUR

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10.4062/biomolther.2016.069

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title = "Reduced autophagy in 5-fluorouracil resistant colon cancer cells",

abstract = "We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.",

keywords = "5-Fluorouracil, Autophagy, Colon cancer, Reactive oxygen species, SNUC5/5-FUR",

author = "Yao, \{Cheng Wen\} and Kang, \{Kyoung Ah\} and Piao, \{Mei Jing\} and Ryu, \{Yea Seong\} and Fernando, \{Pattage Madushan Dilhara Jayatissa\} and Oh, \{Min Chang\} and Park, \{Jeong Eon\} and Kristina Shilnikova and Na, \{Soo Young\} and Jeong, \{Seung Uk\} and Boo, \{Sun Jin\} and Hyun, \{Jin Won\}",

note = "Publisher Copyright: {\textcopyright} 2017 The Korean Society of Applied Pharmacology.",

year = "2017",

month = may,

doi = "10.4062/biomolther.2016.069",

language = "English",

volume = "25",

pages = "315--320",

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T1 - Reduced autophagy in 5-fluorouracil resistant colon cancer cells

AU - Yao, Cheng Wen

AU - Kang, Kyoung Ah

AU - Piao, Mei Jing

AU - Ryu, Yea Seong

AU - Fernando, Pattage Madushan Dilhara Jayatissa

AU - Oh, Min Chang

AU - Park, Jeong Eon

AU - Shilnikova, Kristina

AU - Na, Soo Young

AU - Jeong, Seung Uk

AU - Boo, Sun Jin

AU - Hyun, Jin Won

PY - 2017/5

Y1 - 2017/5

N2 - We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.

AB - We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.

KW - 5-Fluorouracil

KW - Autophagy

KW - Colon cancer

KW - Reactive oxygen species

KW - SNUC5/5-FUR

UR - https://www.scopus.com/pages/publications/85019029696

U2 - 10.4062/biomolther.2016.069

DO - 10.4062/biomolther.2016.069

M3 - Article

AN - SCOPUS:85019029696

SN - 1976-9148

VL - 25

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EP - 320

JO - Biomolecules and Therapeutics

JF - Biomolecules and Therapeutics

IS - 3

ER -

Reduced autophagy in 5-fluorouracil resistant colon cancer cells

Abstract

Bibliographical note

UN SDGs

Keywords

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