Removal of 5T,3T-Untranslated Regions Enhances Erythropoietin Gene Expression in Mammalian Cells

J. H. Park; C. Kim; W. B. Kim; Y. K. Kim; S. Y. Lee; J. M. Yang

Removal of 5T,3T-Untranslated Regions Enhances Erythropoietin Gene Expression in Mammalian Cells

J. H. Park, C. Kim, W. B. Kim, Y. K. Kim, S. Y. Lee, J. M. Yang

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The effect of the 5T- and ST-untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5T-UTR-deleted, 3T-UTR-deleted, and 5T,3T-UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5T,3T-UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5T,3T-UTR-deleted gEpo might be related with the transcription efficiency.

Original language	English
Pages (from-to)	413-416
Number of pages	4
Journal	Molekulyarnaya Biologiya
Volume	35
Issue number	3
State	Published - 2001

Cite this

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title = "Removal of 5T,3T-Untranslated Regions Enhances Erythropoietin Gene Expression in Mammalian Cells",

abstract = "The effect of the 5T- and ST-untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5T-UTR-deleted, 3T-UTR-deleted, and 5T,3T-UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5T,3T-UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5T,3T-UTR-deleted gEpo might be related with the transcription efficiency.",

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year = "2001",

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T1 - Removal of 5T,3T-Untranslated Regions Enhances Erythropoietin Gene Expression in Mammalian Cells

AU - Park, J. H.

AU - Kim, C.

AU - Kim, W. B.

AU - Kim, Y. K.

AU - Lee, S. Y.

AU - Yang, J. M.

PY - 2001

Y1 - 2001

N2 - The effect of the 5T- and ST-untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5T-UTR-deleted, 3T-UTR-deleted, and 5T,3T-UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5T,3T-UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5T,3T-UTR-deleted gEpo might be related with the transcription efficiency.

AB - The effect of the 5T- and ST-untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5T-UTR-deleted, 3T-UTR-deleted, and 5T,3T-UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5T,3T-UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5T,3T-UTR-deleted gEpo might be related with the transcription efficiency.

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M3 - Article

C2 - 11443922

AN - SCOPUS:0035345811

SN - 0026-8984

VL - 35

SP - 413

EP - 416

JO - Molekulyarnaya Biologiya

JF - Molekulyarnaya Biologiya

IS - 3

ER -

Removal of 5T,3T-Untranslated Regions Enhances Erythropoietin Gene Expression in Mammalian Cells

Abstract

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