Run-up of γ-aminobutyric acid(C) responses in catfish retinal cone-horizontal cell axon-terminals is modulated by protein kinase A and C

Using whole-cell voltage-clamp techniques, we investigated the protein kinase modulation of γ-aminobutyric acid(C) (GABA(C))-activated currents relating to run-up regulation in dissociated cone-horizontal cell (HC) axon-terminals from catfish retina. GABA induced an inward chloride current in cells voltage-clamped at -70 mV. With repetitive applications of 10 μM GABA, the peaks of the GABA responses increased up to approximately 135% of the control responses during a period of 10 min. Intracellular application of forskolin, an adenylate cyclase activator, decreased the run-up of GABA(C) responses. H8 dihydrochloride, a cAMP inhibitor, enhanced this run-up to 190% of the control responses. 1-oleoyl-2-acetyl-sn-glycerol, a protein kinase C activator, accelerated the run-up of GABA(C) responses. GF 109203X, a PKC inhibitor, decreased the run-up. These results suggest that retinal GABA(C) responses in cone-HC axon-terminals are modulated by both protein kinase A and C. Copyright (C) 2000 Elsevier Science Ireland Ltd.