SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection

Szu Hsien Sam Wu; Heetak Lee; Réka Szép-Bakonyi; Gabriele Colozza; Ayse Boese; Krista R. Gert; Natalia Hallay; Ji Hyun Lee; Jihoon Kim; Yi Zhu; Margot M. Linssen; Sandra Pilat-Carotta; Peter Hohenstein; Hans Christian Theussl; Andrea Pauli; Bon Kyoung Koo

doi:10.1038/s12276-022-00891-0

SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection

Szu Hsien Sam Wu
, Heetak Lee
, Réka Szép-Bakonyi
, Gabriele Colozza
, Ayse Boese
, Krista R. Gert
, Natalia Hallay
, Ji Hyun Lee
, Jihoon Kim
, Yi Zhu
, Margot M. Linssen
, Sandra Pilat-Carotta
, Peter Hohenstein
, Hans Christian Theussl
, Andrea Pauli
, Bon Kyoung Koo

Department of Medical and Biological Sciences

Institute of Molecular Biotechnology
Medical University of Vienna
Institute for Basic Science
Vienna Biocenter
Leiden University

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.

Original language	English
Pages (from-to)	2188-2199
Number of pages	12
Journal	Experimental and Molecular Medicine
Volume	54
Issue number	12
DOIs	https://doi.org/10.1038/s12276-022-00891-0
State	Published - Dec 2022

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© 2022, The Author(s).

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Wu, S. H. S., Lee, H., Szép-Bakonyi, R., Colozza, G., Boese, A., Gert, K. R., Hallay, N., Lee, J. H., Kim, J., Zhu, Y., Linssen, M. M., Pilat-Carotta, S., Hohenstein, P., Theussl, H. C., Pauli, A., & Koo, B. K. (2022). SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection. Experimental and Molecular Medicine, 54(12), 2188-2199. https://doi.org/10.1038/s12276-022-00891-0

@article{843d22cf2b6b447cb8506bc69ec263ca,

title = "SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection",

abstract = "The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.",

author = "Wu, \{Szu Hsien Sam\} and Heetak Lee and R{\'e}ka Sz{\'e}p-Bakonyi and Gabriele Colozza and Ayse Boese and Gert, \{Krista R.\} and Natalia Hallay and Lee, \{Ji Hyun\} and Jihoon Kim and Yi Zhu and Linssen, \{Margot M.\} and Sandra Pilat-Carotta and Peter Hohenstein and Theussl, \{Hans Christian\} and Andrea Pauli and Koo, \{Bon Kyoung\}",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "10.1038/s12276-022-00891-0",

language = "English",

volume = "54",

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Wu, SHS, Lee, H, Szép-Bakonyi, R, Colozza, G, Boese, A, Gert, KR, Hallay, N, Lee, JH, Kim, J, Zhu, Y, Linssen, MM, Pilat-Carotta, S, Hohenstein, P, Theussl, HC, Pauli, A & Koo, BK 2022, 'SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection', Experimental and Molecular Medicine, vol. 54, no. 12, pp. 2188-2199. https://doi.org/10.1038/s12276-022-00891-0

TY - JOUR

T1 - SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection

AU - Wu, Szu Hsien Sam

AU - Lee, Heetak

AU - Szép-Bakonyi, Réka

AU - Colozza, Gabriele

AU - Boese, Ayse

AU - Gert, Krista R.

AU - Hallay, Natalia

AU - Lee, Ji Hyun

AU - Kim, Jihoon

AU - Zhu, Yi

AU - Linssen, Margot M.

AU - Pilat-Carotta, Sandra

AU - Hohenstein, Peter

AU - Theussl, Hans Christian

AU - Pauli, Andrea

AU - Koo, Bon Kyoung

PY - 2022/12

Y1 - 2022/12

N2 - The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.

AB - The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.

UR - https://www.scopus.com/pages/publications/85143622417

U2 - 10.1038/s12276-022-00891-0

DO - 10.1038/s12276-022-00891-0

M3 - Article

C2 - 36494589

AN - SCOPUS:85143622417

SN - 1226-3613

VL - 54

SP - 2188

EP - 2199

JO - Experimental and Molecular Medicine

JF - Experimental and Molecular Medicine

IS - 12

ER -

SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection

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