Sensitive, selective and resuable electrochemical sensor for mercury(II) based on molecular beacon approach

Donghoon Han, Yang Rae Kim, Jeong Wook Oh, Jong Seung Kim, Hasuck Kim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We have developed a sensitive and selective electrochemical sensor for the detection of mercury(II) ions in aqueous media. This electrochemical sensor is based on the molecular beacon which involves an electroactive, ferrocene-labeled DNA hairpin structure and provides strategically selective binding of thyminethymine mismatch for Hg2+ ions. The modified electrode showed a voltammetric signal due to an one-step redox reaction of the surface-confined ferrocenyl moiety. The "signal-on" upon mercury binding could be attributed to a change in conformation from open structure to restricted hairpin structure. The molecular beacon mercury(II) ion sensor was amenable to regeneration by unfolding the ferrocene-labeled DNA in 10 μM cysteine.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages964-966
Number of pages3
ISBN (Print)9780979806421
StatePublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 1 Nov 20095 Nov 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period1/11/095/11/09

Keywords

  • Electrochemical detection

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