Simultaneous determination of ginsenoside Rb1 and Rg 1 in human plasma by liquid chromatography-mass spectrometry

Hye Young Ji; Hye Won Lee; Hae Kyoung Kim; Hui Hyun Kim; Seung Goo Chang; Dong Hwan Sohn; Jaebaek Kim; Hye Suk Lee

doi:10.1016/j.jpba.2003.12.020

Simultaneous determination of ginsenoside Rb₁ and Rg ₁ in human plasma by liquid chromatography-mass spectrometry

Hye Young Ji
, Hye Won Lee
, Hae Kyoung Kim
, Hui Hyun Kim
, Seung Goo Chang
, Dong Hwan Sohn
, Jaebaek Kim
, Hye Suk Lee

Wonkwang University
Wonpharm Co. Ltd.

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

A liquid chromatographic-mass spectrometric (LC/MS) method for the simultaneous determination of ginsenoside Rb₁ and Rg₁ in human plasma was developed. The method involved the protein precipitation followed by analysis of ginsenoside Rb₁ and Rg₁ in an Atlantis C₁₈ column with the gradient elution of acetonitrile and ammonium formate (10mM, pH 3.0) at a flow rate of 0.2ml/min. The analytes were determined using electrospray negative ionization mass spectrometry in the selected ion monitoring mode. The standard curves for ginsenoside Rb ₁ and Rg₁ were linear over the concentration range of 10.0-1000ng/ml. The lower limit of quantification was 10.0ng/ml using 100μl plasma sample. The coefficient of variation of intra- and inter-day assays for ginsenoside Rb₁ and Rg₁ at three quality control levels ranged from 1.0 to 6.8% and 5.4 to 9.8%, respectively. Ginsenoside Rb ₁ and Rg₁ were stable in blank human plasma at room temperature for 24h and following three freeze-thaw cycles.

Original language	English
Pages (from-to)	207-212
Number of pages	6
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/j.jpba.2003.12.020
State	Published - 1 Apr 2004

Bibliographical note

Funding Information:
This research was supported by a grant (PF 0320401-00) from Plant Diversity Research Center of 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government and Spela Ltd.

Keywords

Ginsenoside Rb
Ginsenoside Rg
LC/MS
Plasma

Access to Document

10.1016/j.jpba.2003.12.020

Cite this

@article{458f12c81c0a46a5a68bbba813a2f205,

title = "Simultaneous determination of ginsenoside Rb1 and Rg 1 in human plasma by liquid chromatography-mass spectrometry",

abstract = "A liquid chromatographic-mass spectrometric (LC/MS) method for the simultaneous determination of ginsenoside Rb1 and Rg1 in human plasma was developed. The method involved the protein precipitation followed by analysis of ginsenoside Rb1 and Rg1 in an Atlantis C18 column with the gradient elution of acetonitrile and ammonium formate (10mM, pH 3.0) at a flow rate of 0.2ml/min. The analytes were determined using electrospray negative ionization mass spectrometry in the selected ion monitoring mode. The standard curves for ginsenoside Rb 1 and Rg1 were linear over the concentration range of 10.0-1000ng/ml. The lower limit of quantification was 10.0ng/ml using 100μl plasma sample. The coefficient of variation of intra- and inter-day assays for ginsenoside Rb1 and Rg1 at three quality control levels ranged from 1.0 to 6.8\% and 5.4 to 9.8\%, respectively. Ginsenoside Rb 1 and Rg1 were stable in blank human plasma at room temperature for 24h and following three freeze-thaw cycles.",

keywords = "Ginsenoside Rb, Ginsenoside Rg, LC/MS, Plasma",

author = "Ji, \{Hye Young\} and Lee, \{Hye Won\} and Kim, \{Hae Kyoung\} and Kim, \{Hui Hyun\} and Chang, \{Seung Goo\} and Sohn, \{Dong Hwan\} and Jaebaek Kim and Lee, \{Hye Suk\}",

note = "Funding Information: This research was supported by a grant (PF 0320401-00) from Plant Diversity Research Center of 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government and Spela Ltd.",

year = "2004",

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doi = "10.1016/j.jpba.2003.12.020",

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T1 - Simultaneous determination of ginsenoside Rb1 and Rg 1 in human plasma by liquid chromatography-mass spectrometry

AU - Ji, Hye Young

AU - Lee, Hye Won

AU - Kim, Hae Kyoung

AU - Kim, Hui Hyun

AU - Chang, Seung Goo

AU - Sohn, Dong Hwan

AU - Kim, Jaebaek

AU - Lee, Hye Suk

N1 - Funding Information: This research was supported by a grant (PF 0320401-00) from Plant Diversity Research Center of 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government and Spela Ltd.

PY - 2004/4/1

Y1 - 2004/4/1

N2 - A liquid chromatographic-mass spectrometric (LC/MS) method for the simultaneous determination of ginsenoside Rb1 and Rg1 in human plasma was developed. The method involved the protein precipitation followed by analysis of ginsenoside Rb1 and Rg1 in an Atlantis C18 column with the gradient elution of acetonitrile and ammonium formate (10mM, pH 3.0) at a flow rate of 0.2ml/min. The analytes were determined using electrospray negative ionization mass spectrometry in the selected ion monitoring mode. The standard curves for ginsenoside Rb 1 and Rg1 were linear over the concentration range of 10.0-1000ng/ml. The lower limit of quantification was 10.0ng/ml using 100μl plasma sample. The coefficient of variation of intra- and inter-day assays for ginsenoside Rb1 and Rg1 at three quality control levels ranged from 1.0 to 6.8% and 5.4 to 9.8%, respectively. Ginsenoside Rb 1 and Rg1 were stable in blank human plasma at room temperature for 24h and following three freeze-thaw cycles.

AB - A liquid chromatographic-mass spectrometric (LC/MS) method for the simultaneous determination of ginsenoside Rb1 and Rg1 in human plasma was developed. The method involved the protein precipitation followed by analysis of ginsenoside Rb1 and Rg1 in an Atlantis C18 column with the gradient elution of acetonitrile and ammonium formate (10mM, pH 3.0) at a flow rate of 0.2ml/min. The analytes were determined using electrospray negative ionization mass spectrometry in the selected ion monitoring mode. The standard curves for ginsenoside Rb 1 and Rg1 were linear over the concentration range of 10.0-1000ng/ml. The lower limit of quantification was 10.0ng/ml using 100μl plasma sample. The coefficient of variation of intra- and inter-day assays for ginsenoside Rb1 and Rg1 at three quality control levels ranged from 1.0 to 6.8% and 5.4 to 9.8%, respectively. Ginsenoside Rb 1 and Rg1 were stable in blank human plasma at room temperature for 24h and following three freeze-thaw cycles.

KW - Ginsenoside Rb

KW - Ginsenoside Rg

KW - LC/MS

KW - Plasma

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