Simultaneous determination of the novel neuroprotective agent KR-31378 and its metabolite KR-31612 using high performance liquid chromatography with tandem mass spectrometry in human plasma

John Kim; Young Ji Hye; Sung Eun Yoo; Ok Kim Sun; Ha Lee Dong; Hong Lim; Suk Lee Hye

doi:10.1007/BF02976938

Simultaneous determination of the novel neuroprotective agent KR-31378 and its metabolite KR-31612 using high performance liquid chromatography with tandem mass spectrometry in human plasma

John Kim, Young Ji Hye, Sung Eun Yoo, Ok Kim Sun, Ha Lee Dong, Hong Lim, Suk Lee Hye

Pharmacy

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

An LC/MS/MS method for the simultaneous determination of a neuroprotective agent for ischemia-reperfusion damage, KR-31378 and its N-acetyl metabolite KR-31612 in human plasma was developed. KR-31378, KR-31612 and the internal standard, KR-31543 were extracted from human plasma by liquid-liquid extraction. A reverse-phase HPLC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-5 mM ammonium formate (55:45, v/v) as mobile phase. The detection of analytes was performed using an electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The lower limits of quantification for KR-31378 and KR-31612 were 2.0 ng/ml. The method showed a satisfactory sensitivity, precision, accuracy, recovery and selectivity.

Original language	English
Pages (from-to)	647-651
Number of pages	5
Journal	Archives of Pharmacal Research
Volume	25
Issue number	5
DOIs	https://doi.org/10.1007/BF02976938
State	Published - 31 Oct 2002

Bibliographical note

Funding Information:
This work was partially supported by the Project from the Center for Biological Modulators (CBM-02-B-8), Ministry of Science and Technology, Republic of Korea.

Keywords

Ischemia
KR-31378
KR-31612
LC/MS/MS
Neuroprotective

Access to Document

10.1007/BF02976938

Cite this

@article{baae7390d48646c993e08ec4b27f08db,

title = "Simultaneous determination of the novel neuroprotective agent KR-31378 and its metabolite KR-31612 using high performance liquid chromatography with tandem mass spectrometry in human plasma",

abstract = "An LC/MS/MS method for the simultaneous determination of a neuroprotective agent for ischemia-reperfusion damage, KR-31378 and its N-acetyl metabolite KR-31612 in human plasma was developed. KR-31378, KR-31612 and the internal standard, KR-31543 were extracted from human plasma by liquid-liquid extraction. A reverse-phase HPLC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-5 mM ammonium formate (55:45, v/v) as mobile phase. The detection of analytes was performed using an electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The lower limits of quantification for KR-31378 and KR-31612 were 2.0 ng/ml. The method showed a satisfactory sensitivity, precision, accuracy, recovery and selectivity.",

keywords = "Ischemia, KR-31378, KR-31612, LC/MS/MS, Neuroprotective",

author = "John Kim and Hye, {Young Ji} and Yoo, {Sung Eun} and Sun, {Ok Kim} and Dong, {Ha Lee} and Hong Lim and Hye, {Suk Lee}",

note = "Funding Information: This work was partially supported by the Project from the Center for Biological Modulators (CBM-02-B-8), Ministry of Science and Technology, Republic of Korea.",

year = "2002",

month = oct,

day = "31",

doi = "10.1007/BF02976938",

language = "English",

volume = "25",

pages = "647--651",

journal = "Archives of Pharmacal Research",

issn = "0253-6269",

publisher = "Pharmaceutical Society of Korea",

number = "5",

}

Simultaneous determination of the novel neuroprotective agent KR-31378 and its metabolite KR-31612 using high performance liquid chromatography with tandem mass spectrometry in human plasma. / Kim, John; Hye, Young Ji; Yoo, Sung Eun et al.
In: Archives of Pharmacal Research, Vol. 25, No. 5, 31.10.2002, p. 647-651.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Simultaneous determination of the novel neuroprotective agent KR-31378 and its metabolite KR-31612 using high performance liquid chromatography with tandem mass spectrometry in human plasma

AU - Kim, John

AU - Hye, Young Ji

AU - Yoo, Sung Eun

AU - Sun, Ok Kim

AU - Dong, Ha Lee

AU - Lim, Hong

AU - Hye, Suk Lee

N1 - Funding Information: This work was partially supported by the Project from the Center for Biological Modulators (CBM-02-B-8), Ministry of Science and Technology, Republic of Korea.

PY - 2002/10/31

Y1 - 2002/10/31

N2 - An LC/MS/MS method for the simultaneous determination of a neuroprotective agent for ischemia-reperfusion damage, KR-31378 and its N-acetyl metabolite KR-31612 in human plasma was developed. KR-31378, KR-31612 and the internal standard, KR-31543 were extracted from human plasma by liquid-liquid extraction. A reverse-phase HPLC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-5 mM ammonium formate (55:45, v/v) as mobile phase. The detection of analytes was performed using an electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The lower limits of quantification for KR-31378 and KR-31612 were 2.0 ng/ml. The method showed a satisfactory sensitivity, precision, accuracy, recovery and selectivity.

AB - An LC/MS/MS method for the simultaneous determination of a neuroprotective agent for ischemia-reperfusion damage, KR-31378 and its N-acetyl metabolite KR-31612 in human plasma was developed. KR-31378, KR-31612 and the internal standard, KR-31543 were extracted from human plasma by liquid-liquid extraction. A reverse-phase HPLC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-5 mM ammonium formate (55:45, v/v) as mobile phase. The detection of analytes was performed using an electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The lower limits of quantification for KR-31378 and KR-31612 were 2.0 ng/ml. The method showed a satisfactory sensitivity, precision, accuracy, recovery and selectivity.

KW - Ischemia

KW - KR-31378

KW - KR-31612

KW - LC/MS/MS

KW - Neuroprotective

UR - http://www.scopus.com/inward/record.url?scp=11244351632&partnerID=8YFLogxK

U2 - 10.1007/BF02976938

DO - 10.1007/BF02976938

M3 - Article

C2 - 12433199

AN - SCOPUS:11244351632

SN - 0253-6269

VL - 25

SP - 647

EP - 651

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

IS - 5

ER -

Simultaneous determination of the novel neuroprotective agent KR-31378 and its metabolite KR-31612 using high performance liquid chromatography with tandem mass spectrometry in human plasma

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this