Sodium nitroprusside selectively induces apoptotic cell death in the outer retina of the rat

W. K. Ju; I. W. Chung; K. Y. Kim; J. S. Gwon; M. Y. Lee; S. J. Oh; M. H. Chun

doi:10.1097/00001756-200112210-00043

Sodium nitroprusside selectively induces apoptotic cell death in the outer retina of the rat

W. K. Ju, I. W. Chung, K. Y. Kim, J. S. Gwon, M. Y. Lee, S. J. Oh, M. H. Chun

Department of Anatomy

The Catholic University of Korea

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Sodium nitroprusside (SNP), an NO donor, was studied for its effects on apoptosis in rat retinal neurons. TUNEL-positive cells were observed in the outer nuclear layer (ONL), but not in the inner retina after SNP treatment. Inner retinal neurons died by necrosis. No photoreceptor cells were found in the ONL after seven days. Immunoblotting confirmed that neurnal NO synthase expression increased up to 5 days (∼170% of control levels), and then declined by 7 days, suggesting that NO induces apoptosis in the ONL, and that inner retinal neurons die by necrosis due to glutamate from damaged photoreceptors.

Original language	English
Pages (from-to)	4075-4079
Number of pages	5
Journal	NeuroReport
Volume	12
Issue number	18
DOIs	https://doi.org/10.1097/00001756-200112210-00043
State	Published - 21 Dec 2001

Keywords

Apoptosis
Necrosis
Nitric oxide
Rat
Retina
Sodium nitroprusside

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10.1097/00001756-200112210-00043

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title = "Sodium nitroprusside selectively induces apoptotic cell death in the outer retina of the rat",

abstract = "Sodium nitroprusside (SNP), an NO donor, was studied for its effects on apoptosis in rat retinal neurons. TUNEL-positive cells were observed in the outer nuclear layer (ONL), but not in the inner retina after SNP treatment. Inner retinal neurons died by necrosis. No photoreceptor cells were found in the ONL after seven days. Immunoblotting confirmed that neurnal NO synthase expression increased up to 5 days (∼170% of control levels), and then declined by 7 days, suggesting that NO induces apoptosis in the ONL, and that inner retinal neurons die by necrosis due to glutamate from damaged photoreceptors.",

keywords = "Apoptosis, Necrosis, Nitric oxide, Rat, Retina, Sodium nitroprusside",

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T1 - Sodium nitroprusside selectively induces apoptotic cell death in the outer retina of the rat

AU - Ju, W. K.

AU - Chung, I. W.

AU - Kim, K. Y.

AU - Gwon, J. S.

AU - Lee, M. Y.

AU - Oh, S. J.

AU - Chun, M. H.

PY - 2001/12/21

Y1 - 2001/12/21

N2 - Sodium nitroprusside (SNP), an NO donor, was studied for its effects on apoptosis in rat retinal neurons. TUNEL-positive cells were observed in the outer nuclear layer (ONL), but not in the inner retina after SNP treatment. Inner retinal neurons died by necrosis. No photoreceptor cells were found in the ONL after seven days. Immunoblotting confirmed that neurnal NO synthase expression increased up to 5 days (∼170% of control levels), and then declined by 7 days, suggesting that NO induces apoptosis in the ONL, and that inner retinal neurons die by necrosis due to glutamate from damaged photoreceptors.

AB - Sodium nitroprusside (SNP), an NO donor, was studied for its effects on apoptosis in rat retinal neurons. TUNEL-positive cells were observed in the outer nuclear layer (ONL), but not in the inner retina after SNP treatment. Inner retinal neurons died by necrosis. No photoreceptor cells were found in the ONL after seven days. Immunoblotting confirmed that neurnal NO synthase expression increased up to 5 days (∼170% of control levels), and then declined by 7 days, suggesting that NO induces apoptosis in the ONL, and that inner retinal neurons die by necrosis due to glutamate from damaged photoreceptors.

KW - Apoptosis

KW - Necrosis

KW - Nitric oxide

KW - Rat

KW - Retina

KW - Sodium nitroprusside

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Sodium nitroprusside selectively induces apoptotic cell death in the outer retina of the rat

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Keywords

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