Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A2 and Δ12-PGJ2

Sang Gun Ahn; Ho Shik Kim; Seong Whan Jeong; Bo Eun Kim; Hyangshuk Rhim; Jae Yong Shim; Jin Woo Kim; Jeong Hwa Lee; In Kyung Kim

doi:10.1038/emm.2002.34

Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A₂ and Δ¹²-PGJ₂

Sang Gun Ahn, Ho Shik Kim, Seong Whan Jeong, Bo Eun Kim, Hyangshuk Rhim, Jae Yong Shim, Jin Woo Kim, Jeong Hwa Lee, In Kyung Kim

Department of Biochemistry

The Catholic University of Korea

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A2 and Δ¹²- PGJ₂ induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca²⁺ ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA₂ and Δ¹²-PGJ₂ in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway. These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.

Original language	English
Pages (from-to)	243-249
Number of pages	7
Journal	Experimental and Molecular Medicine
Volume	34
Issue number	3
DOIs	https://doi.org/10.1038/emm.2002.34
State	Published - 31 Jul 2002

Keywords

Apoptosis
PGA
Sox-4
Δ-PGJ

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10.1038/emm.2002.34

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title = "Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A2 and Δ12-PGJ2",

abstract = "We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A2 and Δ12- PGJ2 induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca2+ ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA2 and Δ12-PGJ2 in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway. These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.",

keywords = "Apoptosis, PGA, Sox-4, Δ-PGJ",

author = "Ahn, \{Sang Gun\} and Kim, \{Ho Shik\} and Jeong, \{Seong Whan\} and Kim, \{Bo Eun\} and Hyangshuk Rhim and Shim, \{Jae Yong\} and Kim, \{Jin Woo\} and Lee, \{Jeong Hwa\} and Kim, \{In Kyung\}",

year = "2002",

month = jul,

day = "31",

doi = "10.1038/emm.2002.34",

language = "English",

volume = "34",

pages = "243--249",

journal = "Experimental and Molecular Medicine",

issn = "1226-3613",

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number = "3",

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T1 - Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A2 and Δ12-PGJ2

AU - Ahn, Sang Gun

AU - Kim, Ho Shik

AU - Jeong, Seong Whan

AU - Kim, Bo Eun

AU - Rhim, Hyangshuk

AU - Shim, Jae Yong

AU - Kim, Jin Woo

AU - Lee, Jeong Hwa

AU - Kim, In Kyung

PY - 2002/7/31

Y1 - 2002/7/31

N2 - We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A2 and Δ12- PGJ2 induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca2+ ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA2 and Δ12-PGJ2 in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway. These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.

AB - We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A2 and Δ12- PGJ2 induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca2+ ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA2 and Δ12-PGJ2 in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway. These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.

KW - Apoptosis

KW - PGA

KW - Sox-4

KW - Δ-PGJ

UR - https://www.scopus.com/pages/publications/0037205967

U2 - 10.1038/emm.2002.34

DO - 10.1038/emm.2002.34

M3 - Article

C2 - 12216117

AN - SCOPUS:0037205967

SN - 1226-3613

VL - 34

SP - 243

EP - 249

JO - Experimental and Molecular Medicine

JF - Experimental and Molecular Medicine

IS - 3

ER -

Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A₂ and Δ¹²-PGJ₂

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