TY - JOUR
T1 - Species-wide genetic incompatibility analysis identifies immune genes as hot spots of deleterious epistasis
AU - Chae, Eunyoung
AU - Bomblies, Kirsten
AU - Kim, Sang Tae
AU - Karelina, Darya
AU - Zaidem, Maricris
AU - Ossowski, Stephan
AU - Martín-Pizarro, Carmen
AU - Laitinen, Roosa A.E.
AU - Rowan, Beth A.
AU - Tenenboim, Hezi
AU - Lechner, Sarah
AU - Demar, Monika
AU - Habring-Müller, Anette
AU - Lanz, Christa
AU - Rätsch, Gunnar
AU - Weigel, Detlef
N1 - Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/12/4
Y1 - 2014/12/4
N2 - Intraspecific genetic incompatibilities prevent the assembly of specific alleles into single genotypes and influence genome- and species-wide patterns of sequence variation. A common incompatibility in plants is hybrid necrosis, characterized by autoimmune responses due to epistatic interactions between natural genetic variants. By systematically testing thousands of F1 hybrids of Arabidopsis thaliana strains, we identified a small number of incompatibility hot spots in the genome, often in regions densely populated by nucleotide-binding domain and leucine-rich repeat (NLR) immune receptor genes. In several cases, these immune receptor loci interact with each other, suggestive of conflict within the immune system. A particularly dangerous locus is a highly variable cluster of NLR genes, DM2, which causes multiple independent incompatibilities with genes that encode a range of biochemical functions, including NLRs. Our findings suggest that deleterious interactions of immune receptors limit the combinations of favorable disease resistance alleles accessible to plant genomes.
AB - Intraspecific genetic incompatibilities prevent the assembly of specific alleles into single genotypes and influence genome- and species-wide patterns of sequence variation. A common incompatibility in plants is hybrid necrosis, characterized by autoimmune responses due to epistatic interactions between natural genetic variants. By systematically testing thousands of F1 hybrids of Arabidopsis thaliana strains, we identified a small number of incompatibility hot spots in the genome, often in regions densely populated by nucleotide-binding domain and leucine-rich repeat (NLR) immune receptor genes. In several cases, these immune receptor loci interact with each other, suggestive of conflict within the immune system. A particularly dangerous locus is a highly variable cluster of NLR genes, DM2, which causes multiple independent incompatibilities with genes that encode a range of biochemical functions, including NLRs. Our findings suggest that deleterious interactions of immune receptors limit the combinations of favorable disease resistance alleles accessible to plant genomes.
UR - http://www.scopus.com/inward/record.url?scp=84920268654&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2014.10.049
DO - 10.1016/j.cell.2014.10.049
M3 - Article
C2 - 25467443
AN - SCOPUS:84920268654
SN - 0092-8674
VL - 159
SP - 1341
EP - 1351
JO - Cell
JF - Cell
IS - 6
ER -