Systematic analysis of attenuated Coxsackievirus expressing a foreign gene as a viral vaccine vector

Dae Sun Kim; Young Joo Cho; Byoung Guk Kim; Su Hae Lee; Jae Hwan Nam

doi:10.1016/j.vaccine.2009.11.017

Systematic analysis of attenuated Coxsackievirus expressing a foreign gene as a viral vaccine vector

Dae Sun Kim, Young Joo Cho, Byoung Guk Kim, Su Hae Lee, Jae Hwan Nam

Department of Medical and Biological Sciences

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Recombinant viruses expressing foreign antigens may provide a convenient vaccine vector capable of inducing preventative immunity. In this study, we explored the capacity of highly attenuated Coxsackievirus B3 (CVB3) to act as a recombinant vector to deliver foreign genes into experimental animals for the purpose of vaccination. The infectious cDNA of highly attenuated CVB3, YYFF, which has been reported previously (Vaccine 27:1974), was used to construct a recombinant YYFF cDNA (YYFF-HCV) by inserting a truncated form of hepatitis C virus (HCV) envelope protein E2 as an antigenic marker immediately upstream from the gene encoding the VP4 capsid protein. In YYFF-HCV-infected HeLa cells, HCV E2 expression was confirmed by immunoblotting and fluorescence microscopy. YYFF-HCV induced the production of antibodies and the cytotoxic T-lymphocyte (CTL) response to HCV E2 in the inoculated mice. Moreover, YYFF-HCV induced no inflammation in the virus-immunized mice. These results demonstrate that recombinant CVB3 expressing a foreign gene can act as a live vaccine vector capable of inducing humoral and cell-mediated immune responses directed against a foreign protein.

Original language	English
Pages (from-to)	1234-1240
Number of pages	7
Journal	Vaccine
Volume	28
Issue number	5
DOIs	https://doi.org/10.1016/j.vaccine.2009.11.017
State	Published - 3 Feb 2010

Bibliographical note

Funding Information:
We are grateful to Drs. R. H. Purcell and S. U. Emerson for the analysis of neutralizing HCV antibodies, and for helpful discussion. This work was supported by grants from the Korea Centers for Disease Control and Prevention ( M-2008-A0082-00002 ), the Gyeonggi Regional Research Center (GRRC) of the Catholic University of Korea , Korea Rural Development Administration and the 2009 Research Fund of the Catholic University of Korea as well as the Ministry of Knowledge Economy (MKE) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology.

Keywords

Attenuation
Coxsackievirus
Viral vaccine vector

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.vaccine.2009.11.017

Cite this

@article{0cdf7c98fa534731ae6d25d91f278a20,

title = "Systematic analysis of attenuated Coxsackievirus expressing a foreign gene as a viral vaccine vector",

abstract = "Recombinant viruses expressing foreign antigens may provide a convenient vaccine vector capable of inducing preventative immunity. In this study, we explored the capacity of highly attenuated Coxsackievirus B3 (CVB3) to act as a recombinant vector to deliver foreign genes into experimental animals for the purpose of vaccination. The infectious cDNA of highly attenuated CVB3, YYFF, which has been reported previously (Vaccine 27:1974), was used to construct a recombinant YYFF cDNA (YYFF-HCV) by inserting a truncated form of hepatitis C virus (HCV) envelope protein E2 as an antigenic marker immediately upstream from the gene encoding the VP4 capsid protein. In YYFF-HCV-infected HeLa cells, HCV E2 expression was confirmed by immunoblotting and fluorescence microscopy. YYFF-HCV induced the production of antibodies and the cytotoxic T-lymphocyte (CTL) response to HCV E2 in the inoculated mice. Moreover, YYFF-HCV induced no inflammation in the virus-immunized mice. These results demonstrate that recombinant CVB3 expressing a foreign gene can act as a live vaccine vector capable of inducing humoral and cell-mediated immune responses directed against a foreign protein.",

keywords = "Attenuation, Coxsackievirus, Viral vaccine vector",

author = "Kim, {Dae Sun} and Cho, {Young Joo} and Kim, {Byoung Guk} and Lee, {Su Hae} and Nam, {Jae Hwan}",

note = "Funding Information: We are grateful to Drs. R. H. Purcell and S. U. Emerson for the analysis of neutralizing HCV antibodies, and for helpful discussion. This work was supported by grants from the Korea Centers for Disease Control and Prevention ( M-2008-A0082-00002 ), the Gyeonggi Regional Research Center (GRRC) of the Catholic University of Korea , Korea Rural Development Administration and the 2009 Research Fund of the Catholic University of Korea as well as the Ministry of Knowledge Economy (MKE) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology.",

year = "2010",

month = feb,

day = "3",

doi = "10.1016/j.vaccine.2009.11.017",

language = "English",

volume = "28",

pages = "1234--1240",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

number = "5",

}

TY - JOUR

T1 - Systematic analysis of attenuated Coxsackievirus expressing a foreign gene as a viral vaccine vector

AU - Kim, Dae Sun

AU - Cho, Young Joo

AU - Kim, Byoung Guk

AU - Lee, Su Hae

AU - Nam, Jae Hwan

N1 - Funding Information: We are grateful to Drs. R. H. Purcell and S. U. Emerson for the analysis of neutralizing HCV antibodies, and for helpful discussion. This work was supported by grants from the Korea Centers for Disease Control and Prevention ( M-2008-A0082-00002 ), the Gyeonggi Regional Research Center (GRRC) of the Catholic University of Korea , Korea Rural Development Administration and the 2009 Research Fund of the Catholic University of Korea as well as the Ministry of Knowledge Economy (MKE) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology.

PY - 2010/2/3

Y1 - 2010/2/3

N2 - Recombinant viruses expressing foreign antigens may provide a convenient vaccine vector capable of inducing preventative immunity. In this study, we explored the capacity of highly attenuated Coxsackievirus B3 (CVB3) to act as a recombinant vector to deliver foreign genes into experimental animals for the purpose of vaccination. The infectious cDNA of highly attenuated CVB3, YYFF, which has been reported previously (Vaccine 27:1974), was used to construct a recombinant YYFF cDNA (YYFF-HCV) by inserting a truncated form of hepatitis C virus (HCV) envelope protein E2 as an antigenic marker immediately upstream from the gene encoding the VP4 capsid protein. In YYFF-HCV-infected HeLa cells, HCV E2 expression was confirmed by immunoblotting and fluorescence microscopy. YYFF-HCV induced the production of antibodies and the cytotoxic T-lymphocyte (CTL) response to HCV E2 in the inoculated mice. Moreover, YYFF-HCV induced no inflammation in the virus-immunized mice. These results demonstrate that recombinant CVB3 expressing a foreign gene can act as a live vaccine vector capable of inducing humoral and cell-mediated immune responses directed against a foreign protein.

AB - Recombinant viruses expressing foreign antigens may provide a convenient vaccine vector capable of inducing preventative immunity. In this study, we explored the capacity of highly attenuated Coxsackievirus B3 (CVB3) to act as a recombinant vector to deliver foreign genes into experimental animals for the purpose of vaccination. The infectious cDNA of highly attenuated CVB3, YYFF, which has been reported previously (Vaccine 27:1974), was used to construct a recombinant YYFF cDNA (YYFF-HCV) by inserting a truncated form of hepatitis C virus (HCV) envelope protein E2 as an antigenic marker immediately upstream from the gene encoding the VP4 capsid protein. In YYFF-HCV-infected HeLa cells, HCV E2 expression was confirmed by immunoblotting and fluorescence microscopy. YYFF-HCV induced the production of antibodies and the cytotoxic T-lymphocyte (CTL) response to HCV E2 in the inoculated mice. Moreover, YYFF-HCV induced no inflammation in the virus-immunized mice. These results demonstrate that recombinant CVB3 expressing a foreign gene can act as a live vaccine vector capable of inducing humoral and cell-mediated immune responses directed against a foreign protein.

KW - Attenuation

KW - Coxsackievirus

KW - Viral vaccine vector

UR - http://www.scopus.com/inward/record.url?scp=74249108852&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2009.11.017

DO - 10.1016/j.vaccine.2009.11.017

M3 - Article

C2 - 19941986

AN - SCOPUS:74249108852

SN - 0264-410X

VL - 28

SP - 1234

EP - 1240

JO - Vaccine

JF - Vaccine

IS - 5

ER -

Systematic analysis of attenuated Coxsackievirus expressing a foreign gene as a viral vaccine vector

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this