TY - JOUR
T1 - The early protective effect of glutamine pretreatment and ischemia preconditioning in renal ischemia-reperfusion injury of rat
AU - Hwang, J. K.
AU - Kim, J. M.
AU - Kim, Y. K.
AU - Kim, S. D.
AU - Park, S. C.
AU - Kim, J. I.
AU - Nam, H. W.
AU - Kim, J.
AU - Moon, I. S.
N1 - Funding Information:
Supported by the fund of Industry-Academic Cooperation Foundation , The Catholic university of Korea in 2009.
PY - 2013/11
Y1 - 2013/11
N2 - Background Heat shock proteins (HSP) play an important role in protecting cells against stress. Methods Using a rat model, we tested the hypothesis that pretreatment with glutamine (Gln) and ischemia preconditioning (IPC) increase the expression of HSP resulting in attenuation of renal ischemia/reperfusion (I/R) injury. Sprague-Dawley rats were randomized into 4 groups [group I, Gln injection (+), IPC (+); group II, Gln injection (+), IPC (-); group III, saline injection (+), IPC (+); group IV, saline injection (+), IPC (-)]. Renal HSP70 expression was determined by Western blotting and kidney function was assessed by blood urea nitrogen and serum creatinine. Renal cross-sections were microscopically examined for tubular necrosis, exfoliation of tubular epithelial cells, cast formation, and monocyte infiltration. Results Gln pretreatment increased intrarenal HSP expression (P =.031). In group I, tubulointerstitial abnormalities were clearly slighter compared with the other groups (P <.001). Conclusion Our experiments suggest that (1) a single dose of Gln could induce HSP expression and (2) IPC could relieve renal I/R injury. In addition, IPC combined with Gln pretreatment had a synergic protective effect against renal I/R injury.
AB - Background Heat shock proteins (HSP) play an important role in protecting cells against stress. Methods Using a rat model, we tested the hypothesis that pretreatment with glutamine (Gln) and ischemia preconditioning (IPC) increase the expression of HSP resulting in attenuation of renal ischemia/reperfusion (I/R) injury. Sprague-Dawley rats were randomized into 4 groups [group I, Gln injection (+), IPC (+); group II, Gln injection (+), IPC (-); group III, saline injection (+), IPC (+); group IV, saline injection (+), IPC (-)]. Renal HSP70 expression was determined by Western blotting and kidney function was assessed by blood urea nitrogen and serum creatinine. Renal cross-sections were microscopically examined for tubular necrosis, exfoliation of tubular epithelial cells, cast formation, and monocyte infiltration. Results Gln pretreatment increased intrarenal HSP expression (P =.031). In group I, tubulointerstitial abnormalities were clearly slighter compared with the other groups (P <.001). Conclusion Our experiments suggest that (1) a single dose of Gln could induce HSP expression and (2) IPC could relieve renal I/R injury. In addition, IPC combined with Gln pretreatment had a synergic protective effect against renal I/R injury.
UR - https://www.scopus.com/pages/publications/84887103179
U2 - 10.1016/j.transproceed.2013.08.028
DO - 10.1016/j.transproceed.2013.08.028
M3 - Article
C2 - 24182785
AN - SCOPUS:84887103179
SN - 0041-1345
VL - 45
SP - 3203
EP - 3208
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 9
ER -