The effect of C3F8 gas on corneal endothelial cells in rabbits

Dong Hyun Jee; Hyun Seung Kim

doi:10.1007/s10384-010-0884-z

The effect of C₃F₈ gas on corneal endothelial cells in rabbits

Department of Ophthalmology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Purpose: To evaluate the ocular effects of perfluoropropane gas (C ₃F₈) in the anterior chamber of rabbit eyes in relation to the gas concentration. Methods: Twenty rabbit eyes were randomly divided into four groups (20%, 15%, and 10% C₃F₈ gas groups, and an air control group). After injection of 0.3 ml of each gas concentration into the anterior chamber, endothelial damage was evaluated by specular microscopic analysis. The main outcome measurements were endothelial cell density, hexagonality, coefficient of variation, corneal thickness, and intraocular pressure (IOP) 1 day, 1 week, and 2 weeks after injection. Two weeks after injection, transmission electronic microscopy was performed to evaluate the structural integrity. Results: The 20% group had a significant decrease in endothelial cell density (P = 0.023), hexagonality (P = 0.031), and increase in corneal thickness (P = 0.045) from baseline to 2 weeks after injection, whereas the other groups exhibited no significant differences. The 20% group had a significant increase in IOP 1 week after injection (P = 0.041). Transmission electron microscopy revealed that the 20% group had a severe flat cell configuration with a damaged intracellular organization. Conclusions: The risk of endothelial cell damage and elevation in IOP was greater in the 20% C ₃F₈ group than in the 15% or 10% C₃F ₈ groups. Therefore, an appropriate concentration of C ₃F₈ gas is needed for different surgical purposes.

Original language	English
Pages (from-to)	602-608
Number of pages	7
Journal	Japanese Journal of Ophthalmology
Volume	54
Issue number	6
DOIs	https://doi.org/10.1007/s10384-010-0884-z
State	Published - Nov 2010

Bibliographical note

Funding Information:
Twenty New Zealand white rabbits weighing between 2 and 2.5 kg were used. The rabbits underwent a preoperative ocular examination consisting of an external examination with a hand-held slit-lamp biomicroscope (Kowa SL-15; Kowa Optimed, Torrance, CA, USA), endothelial cell counts, and determination of hexagonality and the coefficient of variation (CV) with a specular microscope (Non-con-Robo specular microscope; Konan Medical, Hyogo, Japan), and measurement of corneal thickness with an EchoScan US-1800 device (Nidek, Aichi, Japan) and of intraocular pressure (IOP) with a portable tonometer (Tono-Pen; Medtronic Ophthalmics, Jacksonville, FL, USA), all by one examiner. All rabbits were confirmed to have no abnormalities of the cornea preoperatively. This study was approved by the institutional review board of the Animal Research Committee of St Mary’s Hospital. All animals were handled according to the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Vision and Ophthalmology Research.

Keywords

corneal endothelium
gas concentration
perfluoropropane
rabbit

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10.1007/s10384-010-0884-z

Cite this

@article{b412e5c99cdd44ada74e44909e85b390,

title = "The effect of C3F8 gas on corneal endothelial cells in rabbits",

abstract = "Purpose: To evaluate the ocular effects of perfluoropropane gas (C 3F8) in the anterior chamber of rabbit eyes in relation to the gas concentration. Methods: Twenty rabbit eyes were randomly divided into four groups (20\%, 15\%, and 10\% C3F8 gas groups, and an air control group). After injection of 0.3 ml of each gas concentration into the anterior chamber, endothelial damage was evaluated by specular microscopic analysis. The main outcome measurements were endothelial cell density, hexagonality, coefficient of variation, corneal thickness, and intraocular pressure (IOP) 1 day, 1 week, and 2 weeks after injection. Two weeks after injection, transmission electronic microscopy was performed to evaluate the structural integrity. Results: The 20\% group had a significant decrease in endothelial cell density (P = 0.023), hexagonality (P = 0.031), and increase in corneal thickness (P = 0.045) from baseline to 2 weeks after injection, whereas the other groups exhibited no significant differences. The 20\% group had a significant increase in IOP 1 week after injection (P = 0.041). Transmission electron microscopy revealed that the 20\% group had a severe flat cell configuration with a damaged intracellular organization. Conclusions: The risk of endothelial cell damage and elevation in IOP was greater in the 20\% C 3F8 group than in the 15\% or 10\% C3F 8 groups. Therefore, an appropriate concentration of C 3F8 gas is needed for different surgical purposes.",

keywords = "corneal endothelium, gas concentration, perfluoropropane, rabbit",

author = "Jee, \{Dong Hyun\} and Kim, \{Hyun Seung\}",

note = "Funding Information: Twenty New Zealand white rabbits weighing between 2 and 2.5 kg were used. The rabbits underwent a preoperative ocular examination consisting of an external examination with a hand-held slit-lamp biomicroscope (Kowa SL-15; Kowa Optimed, Torrance, CA, USA), endothelial cell counts, and determination of hexagonality and the coefficient of variation (CV) with a specular microscope (Non-con-Robo specular microscope; Konan Medical, Hyogo, Japan), and measurement of corneal thickness with an EchoScan US-1800 device (Nidek, Aichi, Japan) and of intraocular pressure (IOP) with a portable tonometer (Tono-Pen; Medtronic Ophthalmics, Jacksonville, FL, USA), all by one examiner. All rabbits were confirmed to have no abnormalities of the cornea preoperatively. This study was approved by the institutional review board of the Animal Research Committee of St Mary{\textquoteright}s Hospital. All animals were handled according to the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Vision and Ophthalmology Research.",

year = "2010",

month = nov,

doi = "10.1007/s10384-010-0884-z",

language = "English",

volume = "54",

pages = "602--608",

journal = "Japanese Journal of Ophthalmology",

issn = "0021-5155",

publisher = "Springer",

number = "6",

}

TY - JOUR

T1 - The effect of C3F8 gas on corneal endothelial cells in rabbits

AU - Jee, Dong Hyun

AU - Kim, Hyun Seung

N1 - Funding Information: Twenty New Zealand white rabbits weighing between 2 and 2.5 kg were used. The rabbits underwent a preoperative ocular examination consisting of an external examination with a hand-held slit-lamp biomicroscope (Kowa SL-15; Kowa Optimed, Torrance, CA, USA), endothelial cell counts, and determination of hexagonality and the coefficient of variation (CV) with a specular microscope (Non-con-Robo specular microscope; Konan Medical, Hyogo, Japan), and measurement of corneal thickness with an EchoScan US-1800 device (Nidek, Aichi, Japan) and of intraocular pressure (IOP) with a portable tonometer (Tono-Pen; Medtronic Ophthalmics, Jacksonville, FL, USA), all by one examiner. All rabbits were confirmed to have no abnormalities of the cornea preoperatively. This study was approved by the institutional review board of the Animal Research Committee of St Mary’s Hospital. All animals were handled according to the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Vision and Ophthalmology Research.

PY - 2010/11

Y1 - 2010/11

N2 - Purpose: To evaluate the ocular effects of perfluoropropane gas (C 3F8) in the anterior chamber of rabbit eyes in relation to the gas concentration. Methods: Twenty rabbit eyes were randomly divided into four groups (20%, 15%, and 10% C3F8 gas groups, and an air control group). After injection of 0.3 ml of each gas concentration into the anterior chamber, endothelial damage was evaluated by specular microscopic analysis. The main outcome measurements were endothelial cell density, hexagonality, coefficient of variation, corneal thickness, and intraocular pressure (IOP) 1 day, 1 week, and 2 weeks after injection. Two weeks after injection, transmission electronic microscopy was performed to evaluate the structural integrity. Results: The 20% group had a significant decrease in endothelial cell density (P = 0.023), hexagonality (P = 0.031), and increase in corneal thickness (P = 0.045) from baseline to 2 weeks after injection, whereas the other groups exhibited no significant differences. The 20% group had a significant increase in IOP 1 week after injection (P = 0.041). Transmission electron microscopy revealed that the 20% group had a severe flat cell configuration with a damaged intracellular organization. Conclusions: The risk of endothelial cell damage and elevation in IOP was greater in the 20% C 3F8 group than in the 15% or 10% C3F 8 groups. Therefore, an appropriate concentration of C 3F8 gas is needed for different surgical purposes.

AB - Purpose: To evaluate the ocular effects of perfluoropropane gas (C 3F8) in the anterior chamber of rabbit eyes in relation to the gas concentration. Methods: Twenty rabbit eyes were randomly divided into four groups (20%, 15%, and 10% C3F8 gas groups, and an air control group). After injection of 0.3 ml of each gas concentration into the anterior chamber, endothelial damage was evaluated by specular microscopic analysis. The main outcome measurements were endothelial cell density, hexagonality, coefficient of variation, corneal thickness, and intraocular pressure (IOP) 1 day, 1 week, and 2 weeks after injection. Two weeks after injection, transmission electronic microscopy was performed to evaluate the structural integrity. Results: The 20% group had a significant decrease in endothelial cell density (P = 0.023), hexagonality (P = 0.031), and increase in corneal thickness (P = 0.045) from baseline to 2 weeks after injection, whereas the other groups exhibited no significant differences. The 20% group had a significant increase in IOP 1 week after injection (P = 0.041). Transmission electron microscopy revealed that the 20% group had a severe flat cell configuration with a damaged intracellular organization. Conclusions: The risk of endothelial cell damage and elevation in IOP was greater in the 20% C 3F8 group than in the 15% or 10% C3F 8 groups. Therefore, an appropriate concentration of C 3F8 gas is needed for different surgical purposes.

KW - corneal endothelium

KW - gas concentration

KW - perfluoropropane

KW - rabbit

UR - https://www.scopus.com/pages/publications/78751623443

U2 - 10.1007/s10384-010-0884-z

DO - 10.1007/s10384-010-0884-z

M3 - Article

C2 - 21191723

AN - SCOPUS:78751623443

SN - 0021-5155

VL - 54

SP - 602

EP - 608

JO - Japanese Journal of Ophthalmology

JF - Japanese Journal of Ophthalmology

IS - 6

ER -