Topoisomerase II alpha as a universal tumor antigen: Antitumor immunity in murine tumor models and H-2Kb-restricted T cell epitope

Jung Sun Park; Hye Sung Kim; Mi Young Park; Chang Hyun Kim; Yeun Jun Chung; Yong Kil Hong; Tai Gyu Kim

doi:10.1007/s00262-009-0795-3

Topoisomerase II alpha as a universal tumor antigen: Antitumor immunity in murine tumor models and H-2K^b-restricted T cell epitope

Jung Sun Park
, Hye Sung Kim
, Mi Young Park
, Chang Hyun Kim
, Yeun Jun Chung
, Yong Kil Hong
, Tai Gyu Kim

Department of Microbiology

The Catholic University of Korea

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Topoisomerase II alpha (Top2α) is an attractive candidate to be used as a tumor antigen for cancer immunotherapy, because it is abundantly expressed in various tumors and serves as a target for a number of chemotherapeutic agents. In this study, we demonstrated the immunogenicity of Top2α, using dendritic cells (DC) electroporated with RNA encoding the Top2α C-terminus (Top2αCRNA/ DC). Top2αCRNA/DC were able to demonstrate in vitro stimulation of T cells from mice that were previously vaccinated with Top2α-expressing tumor lysate-pulsed DC. Vaccination with Top2αCRNA/DC induced Top2α-specific T cell responses in vivo as well as antitumor effects in various murine tumor models including MC-38, B16F10, and GL26. DC pulsed with p1327 (DSDEDFSGL), defined as an epitope presented by H-2K^b, also induced Top2α-specific immune responses and antitumor effects. Based on these data, Top2α is suggested to be a universal target for cancer immunotherapy.

Original language	English
Pages (from-to)	747-757
Number of pages	11
Journal	Cancer Immunology, Immunotherapy
Volume	59
Issue number	5
DOIs	https://doi.org/10.1007/s00262-009-0795-3
State	Published - May 2010

Bibliographical note

Funding Information:
Acknowledgments This study was supported by a grant from Korea Research Foundation (Grant KRF 2006-005-J00602).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Dendritic cell
Rna electroporation
Topoisomerase ii alpha
Tumor antigen
Vaccine

Access to Document

10.1007/s00262-009-0795-3

Cite this

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title = "Topoisomerase II alpha as a universal tumor antigen: Antitumor immunity in murine tumor models and H-2Kb-restricted T cell epitope",

abstract = "Topoisomerase II alpha (Top2α) is an attractive candidate to be used as a tumor antigen for cancer immunotherapy, because it is abundantly expressed in various tumors and serves as a target for a number of chemotherapeutic agents. In this study, we demonstrated the immunogenicity of Top2α, using dendritic cells (DC) electroporated with RNA encoding the Top2α C-terminus (Top2αCRNA/ DC). Top2αCRNA/DC were able to demonstrate in vitro stimulation of T cells from mice that were previously vaccinated with Top2α-expressing tumor lysate-pulsed DC. Vaccination with Top2αCRNA/DC induced Top2α-specific T cell responses in vivo as well as antitumor effects in various murine tumor models including MC-38, B16F10, and GL26. DC pulsed with p1327 (DSDEDFSGL), defined as an epitope presented by H-2Kb, also induced Top2α-specific immune responses and antitumor effects. Based on these data, Top2α is suggested to be a universal target for cancer immunotherapy.",

keywords = "Dendritic cell, Rna electroporation, Topoisomerase ii alpha, Tumor antigen, Vaccine",

author = "Park, \{Jung Sun\} and Kim, \{Hye Sung\} and Park, \{Mi Young\} and Kim, \{Chang Hyun\} and Chung, \{Yeun Jun\} and Hong, \{Yong Kil\} and Kim, \{Tai Gyu\}",

note = "Funding Information: Acknowledgments This study was supported by a grant from Korea Research Foundation (Grant KRF 2006-005-J00602).",

year = "2010",

month = may,

doi = "10.1007/s00262-009-0795-3",

language = "English",

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AU - Kim, Hye Sung

AU - Park, Mi Young

AU - Kim, Chang Hyun

AU - Chung, Yeun Jun

AU - Hong, Yong Kil

AU - Kim, Tai Gyu

N1 - Funding Information: Acknowledgments This study was supported by a grant from Korea Research Foundation (Grant KRF 2006-005-J00602).

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N2 - Topoisomerase II alpha (Top2α) is an attractive candidate to be used as a tumor antigen for cancer immunotherapy, because it is abundantly expressed in various tumors and serves as a target for a number of chemotherapeutic agents. In this study, we demonstrated the immunogenicity of Top2α, using dendritic cells (DC) electroporated with RNA encoding the Top2α C-terminus (Top2αCRNA/ DC). Top2αCRNA/DC were able to demonstrate in vitro stimulation of T cells from mice that were previously vaccinated with Top2α-expressing tumor lysate-pulsed DC. Vaccination with Top2αCRNA/DC induced Top2α-specific T cell responses in vivo as well as antitumor effects in various murine tumor models including MC-38, B16F10, and GL26. DC pulsed with p1327 (DSDEDFSGL), defined as an epitope presented by H-2Kb, also induced Top2α-specific immune responses and antitumor effects. Based on these data, Top2α is suggested to be a universal target for cancer immunotherapy.

AB - Topoisomerase II alpha (Top2α) is an attractive candidate to be used as a tumor antigen for cancer immunotherapy, because it is abundantly expressed in various tumors and serves as a target for a number of chemotherapeutic agents. In this study, we demonstrated the immunogenicity of Top2α, using dendritic cells (DC) electroporated with RNA encoding the Top2α C-terminus (Top2αCRNA/ DC). Top2αCRNA/DC were able to demonstrate in vitro stimulation of T cells from mice that were previously vaccinated with Top2α-expressing tumor lysate-pulsed DC. Vaccination with Top2αCRNA/DC induced Top2α-specific T cell responses in vivo as well as antitumor effects in various murine tumor models including MC-38, B16F10, and GL26. DC pulsed with p1327 (DSDEDFSGL), defined as an epitope presented by H-2Kb, also induced Top2α-specific immune responses and antitumor effects. Based on these data, Top2α is suggested to be a universal target for cancer immunotherapy.

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KW - Tumor antigen

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