Variety of horizontal cell gap junctions in the rabbit retina

Jiook Cha; Hong Lim Kim; Feng Pan; Myung Hoon Chun; Stephen C. Massey; In Beom Kim

doi:10.1016/j.neulet.2012.01.010

Variety of horizontal cell gap junctions in the rabbit retina

Jiook Cha, Hong Lim Kim, Feng Pan, Myung Hoon Chun, Stephen C. Massey, In Beom Kim

Department of Anatomy

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

In the rabbit retina, there are two types of horizontal cell (HC). The axonless A-type HCs form a coupled network via connexin 50 (Cx50) gap junctions in the outer plexiform layer (OPL). The axon-bearing B-type HCs form two independently coupled networks; the dendritic network via gap junctions consisted of unknown Cx and the axon terminal network via Cx57. The present study was conducted to examine the localization and morphological features of Cx50 and Cx57 gap junctions in rabbit HCs at cellular and subcellular levels. The results showed that each gap junction composed of Cx50 or Cx57 showed distinct features. The larger Cx50 gap junctions were located more proximally than the smaller Cx50 gap junctions. Both Cx50 plaques formed symmetrical homotypic gap junctions, but some small ones had an asymmetrical appearance, suggesting the presence of heterotypic gap junctions or hemichannels. In contrast, Cx57 gap junctions were found in the more distal part of the OPL but never on the axon terminal endings entering the rod spherules, and they were exclusively homotypic. Interestingly, about half of the Cx57 gap junctions appeared to be invaginated. These distinct features of Cx50 and Cx57 gap junctions show the variety of HC gap junctions and may provide insights into the function of different types of HCs.

Original language	English
Pages (from-to)	99-103
Number of pages	5
Journal	Neuroscience Letters
Volume	510
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2012.01.010
State	Published - 29 Feb 2012

Bibliographical note

Funding Information:
This work was funded by Catholic Medical Center Research Foundation (Program year of 2007) to I.-B.K. and NIH ( EY06515 , EY10608 ) to S.C.M.

Keywords

Cx50
Cx57
Electron microscopy
Gap junction
Horizontal cells
Rabbit retina

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10.1016/j.neulet.2012.01.010

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title = "Variety of horizontal cell gap junctions in the rabbit retina",

abstract = "In the rabbit retina, there are two types of horizontal cell (HC). The axonless A-type HCs form a coupled network via connexin 50 (Cx50) gap junctions in the outer plexiform layer (OPL). The axon-bearing B-type HCs form two independently coupled networks; the dendritic network via gap junctions consisted of unknown Cx and the axon terminal network via Cx57. The present study was conducted to examine the localization and morphological features of Cx50 and Cx57 gap junctions in rabbit HCs at cellular and subcellular levels. The results showed that each gap junction composed of Cx50 or Cx57 showed distinct features. The larger Cx50 gap junctions were located more proximally than the smaller Cx50 gap junctions. Both Cx50 plaques formed symmetrical homotypic gap junctions, but some small ones had an asymmetrical appearance, suggesting the presence of heterotypic gap junctions or hemichannels. In contrast, Cx57 gap junctions were found in the more distal part of the OPL but never on the axon terminal endings entering the rod spherules, and they were exclusively homotypic. Interestingly, about half of the Cx57 gap junctions appeared to be invaginated. These distinct features of Cx50 and Cx57 gap junctions show the variety of HC gap junctions and may provide insights into the function of different types of HCs.",

keywords = "Cx50, Cx57, Electron microscopy, Gap junction, Horizontal cells, Rabbit retina",

author = "Jiook Cha and Kim, {Hong Lim} and Feng Pan and Chun, {Myung Hoon} and Massey, {Stephen C.} and Kim, {In Beom}",

note = "Funding Information: This work was funded by Catholic Medical Center Research Foundation (Program year of 2007) to I.-B.K. and NIH ( EY06515 , EY10608 ) to S.C.M. ",

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AU - Kim, Hong Lim

AU - Pan, Feng

AU - Chun, Myung Hoon

AU - Massey, Stephen C.

AU - Kim, In Beom

N1 - Funding Information: This work was funded by Catholic Medical Center Research Foundation (Program year of 2007) to I.-B.K. and NIH ( EY06515 , EY10608 ) to S.C.M.

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N2 - In the rabbit retina, there are two types of horizontal cell (HC). The axonless A-type HCs form a coupled network via connexin 50 (Cx50) gap junctions in the outer plexiform layer (OPL). The axon-bearing B-type HCs form two independently coupled networks; the dendritic network via gap junctions consisted of unknown Cx and the axon terminal network via Cx57. The present study was conducted to examine the localization and morphological features of Cx50 and Cx57 gap junctions in rabbit HCs at cellular and subcellular levels. The results showed that each gap junction composed of Cx50 or Cx57 showed distinct features. The larger Cx50 gap junctions were located more proximally than the smaller Cx50 gap junctions. Both Cx50 plaques formed symmetrical homotypic gap junctions, but some small ones had an asymmetrical appearance, suggesting the presence of heterotypic gap junctions or hemichannels. In contrast, Cx57 gap junctions were found in the more distal part of the OPL but never on the axon terminal endings entering the rod spherules, and they were exclusively homotypic. Interestingly, about half of the Cx57 gap junctions appeared to be invaginated. These distinct features of Cx50 and Cx57 gap junctions show the variety of HC gap junctions and may provide insights into the function of different types of HCs.

AB - In the rabbit retina, there are two types of horizontal cell (HC). The axonless A-type HCs form a coupled network via connexin 50 (Cx50) gap junctions in the outer plexiform layer (OPL). The axon-bearing B-type HCs form two independently coupled networks; the dendritic network via gap junctions consisted of unknown Cx and the axon terminal network via Cx57. The present study was conducted to examine the localization and morphological features of Cx50 and Cx57 gap junctions in rabbit HCs at cellular and subcellular levels. The results showed that each gap junction composed of Cx50 or Cx57 showed distinct features. The larger Cx50 gap junctions were located more proximally than the smaller Cx50 gap junctions. Both Cx50 plaques formed symmetrical homotypic gap junctions, but some small ones had an asymmetrical appearance, suggesting the presence of heterotypic gap junctions or hemichannels. In contrast, Cx57 gap junctions were found in the more distal part of the OPL but never on the axon terminal endings entering the rod spherules, and they were exclusively homotypic. Interestingly, about half of the Cx57 gap junctions appeared to be invaginated. These distinct features of Cx50 and Cx57 gap junctions show the variety of HC gap junctions and may provide insights into the function of different types of HCs.

KW - Cx50

KW - Cx57

KW - Electron microscopy

KW - Gap junction

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Variety of horizontal cell gap junctions in the rabbit retina

Abstract

Bibliographical note

Keywords

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